detection of dissolved lactose employing an optofluidic micro-system检测的溶解乳糖雇佣一个optofluidic micro-system.pdfVIP

detection of dissolved lactose employing an optofluidic micro-system检测的溶解乳糖雇佣一个optofluidic micro-system.pdf

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detection of dissolved lactose employing an optofluidic micro-system检测的溶解乳糖雇佣一个optofluidic micro-system

Diagnostics 2012, 2, 97-106; doi:10.3390/diagnostics2040097 OPEN ACCESS diagnostics ISSN 2075-4418 /journal/diagnostics Article Detection of Dissolved Lactose Employing an Optofluidic Micro-System Emanuel Weber , Franz Keplinger and Michael J. Vellekoop Institute for Microsensors, Actuators and Systems (IMSAS), Microsystems Center Bremen (MCB), University of Bremen, Otto-Hahn-Allee NW1, 28359 Bremen, Germany; E-Mail: mvellekoop@imsas.uni-bremen.de Institute of Sensor and Actuator Systems, Vienna University of Technology, Gusshausstrasse 27-29, E366, 1040 Vienna, Austria; E-Mail: franz.keplinger@tuwien.ac.at Author to whom correspondence should be addressed; E-Mail: eweber@imsas.uni-bremen.de; Tel.: +49-421-218-62655; Fax: +49-421-218-98-62655. Received: 2 October 2012; in revised form: 26 November 2012 / Accepted: 3 December 2012 / Published: 6 December 2012 Abstract: In this work, a novel optofluidic sensor principle is employed for a non-invasive and label-free characterization of lactose containing liquid samples. Especially for medicine and food industry, a simple, fast and accurate determination of the amount of lactose in various products is highly desirable. The presented system exploits the impact of dissolved molecules on the refractive index for sample characterization. On the optofluidic chip, a microfluidic channel filled with the analyte is hit by slightly diverging laser light. The center incident angle of the beam on-chip is set close to the critical angle for total internal refl

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