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detailed transcriptome description of the neglected cestode taenia multiceps详细的转录组的描述被忽视的绦虫绦虫曲草
Detailed Transcriptome Description of the Neglected Cestode Taenia multiceps 1 1 1 1 1 1 1 Xuhang Wu , Yan Fu , Deying Yang , Runhui Zhang , Wanpeng Zheng , Huaming Nie , Yue Xie , 1 1 1 1 2 1 Ning Yan , Guiying Hao , Xiaobin Gu , Shuxian Wang , Xuerong Peng , Guangyou Yang * 1 Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China, 2 Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an, China Abstract Background: The larval stage of Taenia multiceps, a global cestode, encysts in the central nervous system (CNS) of sheep and other livestock. This frequently leads to their death and huge socioeconomic losses, especially in developing countries. This parasite can also cause zoonotic infections in humans, but has been largely neglected due to a lack of diagnostic techniques and studies. Recent developments in next-generation sequencing provide an opportunity to explore the transcriptome of T. multiceps. Methodology/Principal Findings: We obtained a total of 31,282 unigenes (mean length 920 bp) using Illumina paired-end sequencing technology and a new Trinity de novo assembler without a referenced genome. Individual transcription molecules were determined by sequence-based annotations and/or domain-based annotations against public databases (Nr, UniprotKB/Swiss-Prot, COG, KEGG, UniProtKB/TrEMBL, InterPro and Pfam). We identified 26,110 (83.47%) unigenes and inferred 20,896 (66.8%) coding sequences (CDS). Further comparative transcripts analy
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