detailed structural analysis of lipids directly on tissue specimens using a maldi-spiraltof-reflectron tof mass spectrometer直接的详细结构分析脂质组织标本使用maldi-spiraltof-reflectron tof质谱计.pdfVIP

Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer 1 2 2 3 Shuichi Shimma *, Ayumi Kubo , Takaya Satoh , Michisato Toyoda 1Venture Business Laboratory, Osaka University, Suita, Osaka, Japan, 2 JEOL Ltd., Akishima, Tokyo, Japan, 3 Project Research Center for Fundamental Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan Abstract Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer ‘‘SpiralTOF (STOF)’’, a collision cell, and an offset parabolic reflectron (RTOF). The features of this system are high precursor ion selectivity due to a 17-m flight path length in STOF and elimination of post-source decay (PSD) ions. The acceleration energy is 20 keV, so that high-energy collision-induced dissociation (HE-CID) is possible. Elimination of PSD ions allows observation of the product ions inherent to the HE-CID process. By using this tandem TOF instrument, the product ion spectrum of lipids provided detailed structural information of fatty acid residues. Citation: Shimma S, Kubo A, Satoh T, Toyoda M (2012) Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer. PLoS ONE 7(5): e37107. doi:10.1371/journal.pone.0037107 Editor: Akos Vertes, The George Washington University, United States of America Received January 1, 2012; Accepted April 18, 2012; Published May 18, 2012 Copyright: 2012 Shimma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, dis