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Desynchronisation of Glycolytic Oscillations in Yeast Cell Populations ´ 1,2 2 2 1 Andre Weber , Yury Prokazov , Werner Zuschratter , Marcus J. B. Hauser * 1 Biophysics Group, Institute of Experimental Physics, Otto von Guericke University, Magdeburg, Germany, 2 Special Laboratory for Electron and Laserscanning Microscopy, Leibniz Institute for Neurobiology, Magdeburg, Germany Abstract Glycolytic oscillations of intact yeast cells of the strain Saccharomyces carlsbergensis were investigated at both the levels of cell populations and of individual cells. Individual cells showed glycolytic oscillations even at very low cell densities (e.g. 1.0 |105 cells/ml). By contrast, the collective behaviour on the population level was cell density-dependent: at high cell densities it is oscillatory, but below the threshold density of 1.0 |106 cells/ml the collective dynamics becomes quiescent. We demonstrate that the transition in the collective dynamics is caused by the desynchronisation of the oscillations of individual cells. This is characteristic for a Kuramoto transition. Spatially resolved measurements at low cell densities revealed that even cells that adhere to their neighbours oscillated with their own, independent frequencies and phases. Citation: Weber A, Prokazov Y, Zuschratter W, Hauser MJB (2012) Desynchronisation of Glycolytic Oscillations in Yeast Cell Populations. PLoS ONE 7(9): e43276. doi:10.1371/journal.pone.0043276 ¨ Editor: Jurgen Kurths, Humboldt University, Germany Received May 29, 2012; Accepted July 18, 2012; Published September 11, 2012 Copyright: 2012 Weber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Lice