有哪些信誉好的足球投注网站cytotoxicity analysis of three bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells三个苏云金杆菌无性系种群的细胞毒性分析。.pdfVIP
- 1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
cytotoxicity analysis of three bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells三个苏云金杆菌无性系种群的细胞毒性分析。
Cytotoxicity Analysis of Three Bacillus thuringiensis Subsp. israelensis d-Endotoxins towards Insect and Mammalian Cells ˆ 1 ´ 1 2 Roberto Franco Teixeira Correa , Daniel Mendes Pereira Ardisson-Araujo , Rose Gomes Monnerat , Bergmann Morais Ribeiro1* ´ ´ ´ ´ 1 Departamento de Biologia Celular, Universidade de Brasılia, Brasılia, Distrito Federal, Brazil, 2 Embrapa – Recursos Geneticos e Biotecnologia, C.P. 02373, Brasılia, Distrito Federal, Brazil Abstract Three members of the d-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell lines. Both Cry4Aa and Cry11A toxins, activated with either trypsin or Spodoptera frugiperda gastric juice (GJ), resulted in different cleavage patterns for the activated toxins as seen by SDS. The GJ-processed proteins were not cytotoxic to insect cell cultures. On the other hand, the combination of the trypsin- activated Cry4Aa and Cry11A toxins yielded the highest levels of cytotoxicity to all insect cells tested. The combination of activated Cyt2Ba and Cry11A also showed higher toxic activity than that of toxins activated individually. When activated Cry4Aa, Cry11A and Cyt2Ba were used simultaneously in the same assay a decrease in toxic activity was observed in all insect cells tested. No toxic effect was observed for the trypsin-activated Cry toxins in mammalian cells, but activated Cyt2Ba was toxic t
您可能关注的文档
- core site-moiety maps reveal inhibitors and binding mechanisms of orthologous proteins by screening compound libraries核心site-moiety地图揭示同源蛋白的抑制剂和绑定机制筛选化合物库.pdf
- corneal transduction by intra-stromal injection of aav vectors in vivo in the mouse and ex vivo in human explants角膜转导intra-stromal注入aav载体体内的老鼠和人类外植体体外.pdf
- corneal alterations during combined therapy with cyclodextrinallopregnanolone and miglustat in a knock-out mouse model of npc1 disease在联合治疗角膜改变cyclodextrinallopregnanolone和miglustat淘汰赛npc1疾病的小鼠模型.pdf
- coronavirus spike protein inhibits host cell translation by interaction with eif3f冠状病毒突起蛋白通过与eif3f交互抑制宿主细胞的翻译.pdf
- corpus callosum morphology in capuchin monkeys is influenced by sex and handedness在卷尾猴胼胝体形态是影响性和构型.pdf
- correcting the bias of empirical frequency parameter estimators in codon models纠正偏差的经验频率参数估计在密码子模型.pdf
- correcting for mortality among patients lost to follow up on antiretroviral therapy in south africa a cohort analysis校正死亡率患者失去跟进抗逆转录病毒疗法在南非一群分析.pdf
- correction hu, x.; sun, f. and zou, y. estimation of state of charge of a lithium-ion battery pack for electric vehicles using an adaptive luenberger observer. energies 2010, 3, 1586–1603校正,x;.pdf
- correction jia, c. and batterman, s. a critical review of naphthalene sources and exposures relevant to indoor and outdoor air. int. j. environ. res. public health 2010, 7, 2903-2939修正贾庆林、c,batterman萘的关键评论来源和风险相关的室内和室外空气。.pdf
- coronin is a component of the endocytic collar of hyphae of neurospora crassa and is necessary for normal growth and morphogenesiscoronin是一个组件的内吞作用的衣领粗糙脉孢菌菌丝和正常生长和形态发生是必要的.pdf
- cytotoxic responses and potential respiratory health effects of carbon and carbonaceous nanoparticulates in the paso del norte airshed environment细胞毒性反应和呼吸健康的潜在影响碳和碳质nanoparticulates paso del norte空气域环境.pdf
- cytoplasmic relaxation of active eph controls ephrin shedding by adam10细胞质的活跃弗管制放松ephrin adam10脱落.pdf
- cytotoxicity of superoxide dismutase 1 in cultured cells is linked to zn2+ chelation在培养细胞超氧化物歧化酶1的细胞毒性与zn2 +螯合.pdf
- cytotoxicity of the ascidian cystodytes dellechiajei against tumor cells and study of the involvement of associated microbiota in the production of cytotoxic compounds细胞毒性的海鞘类cystodytes dellechiajei对肿瘤细胞和研究相关的微生物群的参与生产的细胞毒性的化合物.pdf
- d-(+)-galactose-conjugated single-walled carbon nanotubes as new chemical probes for electrochemical biosensors for the cancer marker galectin-3d -(+)-galactose-conjugated单壁碳纳米管作为癌症的新化学探针电化学生物传感器galectin-3标志.pdf
- cytotoxicity on human cancer cells of ophidiacerebrosides isolated from the african starfish narcissia canariensis在人类癌症细胞的细胞毒性ophidiacerebrosides孤立的非洲海星narcissia canariensis.pdf
- cytotoxicity of phenol red in toxicity assays for carbon nanoparticles细胞毒性的酚红为碳纳米粒子毒性分析.pdf
- cytotoxicity of cd56bright nk cells towards autologous activated cd4+ t cells is mediated through nkg2d, lfa-1 and trail and dampened via cd94nkg2acd56bright nk细胞的细胞毒性对自体激活通过nkg2d cd4 + t细胞是介导,通过cd94nkg2a lfa-1小道和抑制.pdf
- daf-2 signaling modifies mutant sod1 toxicity in c. elegansdaf - 2信号修改sod1基因突变在秀丽隐杆线虫毒性.pdf
- daf-2insulin-like signaling in c. elegans modifies effects of dietary restriction and nutrient stress on aging, stress and growthdaf-2insulin-like信号在秀丽隐杆线虫修改限制饮食和营养应激对老化的影响,压力和增长.pdf
文档评论（0）