covr-controlled global regulation of gene expression in streptococcus mutanscovr-controlled全球监管的变形链球菌基因表达.pdfVIP

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covr-controlled global regulation of gene expression in streptococcus mutanscovr-controlled全球监管的变形链球菌基因表达.pdf

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covr-controlled global regulation of gene expression in streptococcus mutanscovr-controlled全球监管的变形链球菌基因表达

CovR-Controlled Global Regulation of Gene Expression in Streptococcus mutans 1,2 1 1¤ 3 1 Alexander Dmitriev , Saswat S. Mohapatra , Patrick Chong , Melody Neely , Saswati Biswas , Indranil Biswas1* 1 Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America, 2 Department of Molecular Microbiology, Institute of Experimental Medicine, Saint-Petersburg, Russia, 3 Department of Microbiology and Immunology, Wayne State School of Medicine, Detroit, Michigan, United States of America Abstract CovR/S is a two-component signal transduction system (TCS) that controls the expression of various virulence related genes in many streptococci. However, in the dental pathogen Streptococcus mutans, the response regulator CovR appears to be an orphan since the cognate sensor kinase CovS is absent. In this study, we explored the global transcriptional regulation by CovR in S. mutans. Comparison of the transcriptome profiles of the wild-type strain UA159 with its isogenic covR deleted strain IBS10 indicated that at least 128 genes (,6.5% of the genome) were differentially regulated. Among these genes, 69 were down regulated, while 59 were up regulated in the IBS10 strain. The S. mutans CovR regulon included competence genes, virulence related genes, and genes encoded within two genomic islands (GI). Genes encoded by the GI TnSmu2 were found to be dramatically reduced in IBS10, while genes encoded by the GI TnSmu1 were up regulated in the mutant. The microarray data were further confirmed by real-time RT-PCR analyses. Furthermore, direct regulation of some of the differentially expressed genes was demonstrated by electrophoreti