contrasting transcriptional responses of a virulent and an attenuated strain of mycobacterium tuberculosis infecting macrophages对比转录反应结核分枝杆菌的毒性和减毒株感染巨噬细胞.pdfVIP

Contrasting Transcriptional Responses of a Virulent and an Attenuated Strain of Mycobacterium tuberculosis Infecting Macrophages 3.¤ 5. 5 3 2 2 Alice H. Li , Simon J. Waddell , Jason Hinds , Chad A. Malloff , Manjeet Bains , Robert E. Hancock , 3 5. 1,2,3,4 . Wan L. Lam , Philip D. Butcher , Richard W. Stokes * 1 Department of Paediatrics, University of British Columbia, Vancouver, Canada, 2 Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada, 3 Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada, 4 Division of Infectious Diseases, British Columbia’s Children’s Hospital, Vancouver, Canada, 5 Division of Cellular Molecular Medicine, Department of Medical Microbiology, Centre for Infection, St. George’s University of London, London, United Kingdom Abstract Background: H37Rv and H37Ra are well-described laboratory strains of Mycobacterium tuberculosis derived from the same parental strain, H37, that show dramatically different pathogenic phenotypes. Methodology/Principal Findings: In this study, the transcriptomes of the two strains during axenic growth in broth and during intracellular growth within murine bone-marrow macrophages were compared by whole genome expression profiling. We identified and compared adaptations of either strain upon encountering an intracellular environment, and also contrasted the transcriptomes of the two strains while inside macrophages. In the former comparison, both strains induced genes that would facilitate intracellular survival including t