conjunctival reconstruction with progenitor cell-derived autologous epidermal sheets in rhesus monkey结膜重建与祖细胞衍生自体表皮负债表恒河猴.pdfVIP

Conjunctival Reconstruction with Progenitor Cell- Derived Autologous Epidermal Sheets in Rhesus Monkey 1 2 1 1 1 1 1 Rong Lu , Xinchun Zhang , Danping Huang , Bing Huang , Nan Gao , Zhichong Wang , Jian Ge * 1 State Key laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China, 2 Department of Prosthodontics, Hospital of Stomatology, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, China Abstract Severe ocular surface diseases are some of the most challenging problems that the clinician faces today. Conventional management is generally unsatisfactory, and the long-term ocular consequences of these conditions are devastating. It is significantly important to find a substitute for conjunctival epithelial cells. This study was to explore the possibility of progenitor cell-derived epidermal sheets on denuded amniotic membrane to reconstruct ocular surface of conjunctiva damaged monkeys. We isolated epidermal progenitor cells of rhesus monkeys by type IV collagen adhesion, and then expanded progenitor cell-derived epidermal sheets on denuded amniotic membrane ex vivo. At 3 weeks after the conjunctiva injury, the damaged ocular surface of four monkeys was surgically reconstructed by transplanting the autologous cultivated epidermal progenitor cells. At 2 weeks after surgery, transplants were removed and examined with Hematoxylin-eosin staining, Periodic acid Schiff staining, immunofluorescent staining, scanning and transmission electron microscopy. Histological examination of transplanted sheets revealed that the cell sheets were healthy al