conical tomography of a ribbon synapse structural evidence for vesicle fusion锥形断层带状突触结构的囊泡融合的证据.pdfVIP

Conical Tomography of a Ribbon Synapse: Structural Evidence for Vesicle Fusion 1,2 1 2 3 Guido A. Zampighi , Cataldo Schietroma , Lorenzo M. Zampighi , Michael Woodruff , Ernest M. Wright2, Nicholas C. Brecha1,4,5,6* 1 Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California Los Angeles, Los Angeles, California, United States of America, 2 Department of Physiology, David Geffen School of Medicine at UCLA, University of California Los Angeles, Los Angeles, California, United States of America, 3 Integrative Biology and Physiology, College of Letters and Science, University of California Los Angeles, Los Angeles, California, United States of America, 4 Department of Medicine, David Geffen School of Medicine at UCLA, University of California Los Angeles, Los Angeles, California, United States of America, 5 Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, University of California Los Angeles, Los Angeles, California, United States of America, 6 Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California, United States of America Abstract To characterize the sites of synaptic vesicle fusion in photoreceptors, we evaluated the three-dimensional structure of rod spherules from mice exposed to steady bright light or dark-adapted for periods ranging from 3 to 180 minutes using conical electron tomography. Conical tilt series from mice retinas were reconstructed using the weighted back projection algorithm, refined by projection matching and analyzed using semiautomatic density segmentation. In the light, rod spherules contained ,470 vesicles that were hemi-fused and ,187 vesicles that were fully fused (om