有哪些信誉好的足球投注网站comprehensive identification of salmonella enterica serovar typhimurium genes required for infection of balbc mice全面识别所需的鼠伤寒沙门氏菌血清型基因感染balbc老鼠.pdfVIP
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comprehensive identification of salmonella enterica serovar typhimurium genes required for infection of balbc mice全面识别所需的鼠伤寒沙门氏菌血清型基因感染balbc老鼠
Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice 1. 1. 1 1 1 Roy R. Chaudhuri , Sarah E. Peters , Stephen J. Pleasance , Helen Northen , Chrissie Willers , Gavin K. 1 1¤a 2¤b 2¤c 2¤d 3 Paterson , Danielle B. Cone , Andrew G. Allen , Paul J. Owen , Gil Shalom , Dov J. Stekel , Ian G. Charles4, Duncan J. Maskell1* 1 Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom, 2 Arrow Therapeutics Ltd., London, United Kingdom, 3 Centre for Systems Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom, 4 Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom Abstract Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH), has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of the t
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