blocking of connexin-mediated communication promotes neuroprotection during acute degeneration induced by mechanical trauma阻塞connexin-mediated沟通促进神经保护期间急性变性引起的机械损伤.pdfVIP

Blocking of Connexin-Mediated Communication Promotes Neuroprotection during Acute Degeneration Induced by Mechanical Trauma 1,2 1,2 3 2 Vera Paschon , Guilherme Shigueto Vilar Higa , Rodrigo Ribeiro Resende , Luiz Roberto G . Britto , Alexandre Hiroaki Kihara1,2* ´ ˜ ´ ˜ ˜ ´ ˜ 1 Nucleo de Cognic¸ao e Sistemas Complexos, Centro de Matematica, Computac¸ao e Cognic¸ao, Universidade Federal do ABC, Santo Andre, Sao Paulo, Brazil, ´ ˆ ´ ˜ ˜ ˜ ´ 2 Departamento de Fisiologia e Biofısica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil, 3 Departamento de Bioquımica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil Abstract Accruing evidence indicates that connexin (Cx) channels in the gap junctions (GJ) are involved in neurodegeneration after injury. However, studies using KO animal models endowed apparently contradictory results in relation to the role of coupling in neuroprotection. We analyzed the role of Cx-mediated communication in a focal lesion induced by mechanical trauma of the retina, a model that allows spatial and temporal definition of the lesion with high reproducibility, permitting visualization of the focus, penumbra and adjacent areas. Cx36 and Cx43 exhibited distinct gene expression and protein levels throughout the neurodegeneration progress.