bisphenol a-mediated suppression of lpl gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells双酚a lpl基因表达抑制甘油三酯积累一个调解的抑制脂肪形成的人类成体干细胞的分化.pdfVIP

Bisphenol A-Mediated Suppression of LPL Gene Expression Inhibits Triglyceride Accumulation during Adipogenic Differentiation of Human Adult Stem Cells 1 2 1 3 Chris Linehan *, Sanjeev Gupta , Afshin Samali , Lynn O’Connor * 1 Department of Biochemistry, Faculty of Life Sciences, National University of Ireland Galway, Galway, Ireland, 2 Department of Pathology, School of Medicine, Clinical Science Institute, National University of Ireland Galway, Galway, Ireland, 3 Department of Pharmacology, School of Medicine, National University of Ireland Galway, Galway, Ireland Abstract The endocrine disrupting chemical, bisphenol A (BPA), has been shown to accelerate the rate of adipogenesis and increase the amount of triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. The objective of this study was to investigate if that observation is mirrored in human primary cells. Here we investigated the effect of BPA on adipogenesis in cultured human primary adult stem cells. Continuous exposure to BPA throughout the 14 days of differentiation dramatically reduced triglyceride accumulation and suppressed gene transcription of the lipogenic enzyme, lipoprotein lipase (LPL). Results presented in the present study show for the first time that BPA can reduce triglyceride accumulation during adipogenesis by attenuating the expression of LPL gene transcription. Also, by employing image cytometric analysis rather than conventional Oil red O staining techniques we show that BPA regulates triglyceride accumulation in a manner which does not appear to effect adipogenesis per se. Citation: Linehan C, Gupta S, Samali A, O’Connor L (2012) Bisphenol A-Mediated Suppression of LPL Gene Expression Inhibits Triglyceride Accumulation during Adipogenic Differ