bisbibenzyls, a new type of antifungal agent, inhibit morphogenesis switch and biofilm formation through upregulation of dpp3 in candida albicansbisbibenzyls,一种新型的抗真菌剂,抑制形态发生开关和生物膜的形成通过upregulation dpp3白色念珠菌.pdfVIP

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bisbibenzyls, a new type of antifungal agent, inhibit morphogenesis switch and biofilm formation through upregulation of dpp3 in candida albicansbisbibenzyls,一种新型的抗真菌剂,抑制形态发生开关和生物膜的形成通过upregulation dpp3白色念珠菌.pdf

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bisbibenzyls, a new type of antifungal agent, inhibit morphogenesis switch and biofilm formation through upregulation of dpp3 in candida albicansbisbibenzyls,一种新型的抗真菌剂,抑制形态发生开关和生物膜的形成通过upregulation dpp3白色念珠菌

Bisbibenzyls, a New Type of Antifungal Agent, Inhibit Morphogenesis Switch and Biofilm Formation through Upregulation of DPP3 in Candida albicans 1 1 1 2 2 1 Li Zhang , Wenqiang Chang , Bin Sun , Matthias Groh , Andreas Speicher , Hongxiang Lou * ¨ 1 School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China, 2 Chemistry – Organic Chemistry, Saarland University, Saarbrucken, Germany Abstract The yeast-to-hypha transition plays a crucial role in the pathogenesis of C. albicans. Farnesol, a quorum sensing molecule (QSM) secreted by the fungal itself, could prevent the formation of hyphae and subsequently lead to the defect of biofilm formation. The DPP3, encoding phosphatase, is a key gene in regulating farnesol synthesis. In this study, we screened 24 bisbibenzyls and 2 bibenzyls that were isolated from bryophytes or chemically synthesized by using CLSI method for antifungal effect. Seven bisbibenzyls were found to have antifungal effects with IC80 less than 32 mg/ml, and among them, plagiochin F, isoriccardin C and BS-34 were found to inhibit the hyphae and biofilm formation of C. albicans in a dose- dependent manner. To uncover the underlying relationship between morphogenesis switch and QSM formation, we measured the farnesol production by HPLC-MS and quantified Dpp3 expression by detecting the fluorescent intensity of green fluorescent protein tagged strain using Confocal Laser Scanning microscopy and Multifunction Microplate Reader. The DPP3 transcripts were determined by real-time PCR. The data indicated that the bisbibenzyls exerted antifungal ef