μfbi a microfluidic bead-based immunoassay for multiplexed detection of proteins from a μl sample volumeμfbi多路检测的微流控bead-based免疫测定蛋白质μl样本体积.pdfVIP

mFBI: A Microfluidic Bead-Based Immunoassay for Multiplexed Detection of Proteins from a mL Sample Volume 1 1,2 1 1 1 Xiaobo Yu , Michael Hartmann , Quan Wang , Oliver Poetz , Nicole Schneiderhan-Marra , Dieter 1,4 1 1,3 Stoll , Cornelia Kazmaier , Thomas O. Joos * ¨ 1 Natural and Medical Sciences Institute (NMI), University of Tubingen, Reutlingen, Germany, 2 Department of Analytical Chemistry, School of Chemical Science and Engineering, Royal Institute of Technology, Stockholm, Sweden, 3 Experimentelle and Diagnostische Immunologie (EDI) GmbH, Reutlingen, Germany, 4 Hochschule Albstadt-Sigmaringen, Sigmaringen, Germany Abstract Background: Over the last ten years, miniaturized multiplexed immunoassays have become robust, reliable research tools that enable researchers to simultaneously determine a multitude of parameters. Among the numerous analytical protein arrays available, bead-based assay systems have evolved into a key technology that enables the quantitative protein profiling of biological samples whilst requiring only a minimal amount of sample material. Methodology/Principal Findings: A microfluidic bead-based immunoassay, mFBI, was developed to perform bead-based multiplexed sandwich immunoassays in a capillary. This setup allows the simultaneous detection of several parameters and only requires 200 ng of tissue lysate in a 1 mL assay volume. In addition, only 1 mL of detection antibodies and 1 mL of the reporter molecule Streptavidin-Phycoerythrin were required. The mFBI was used to compare the expression