an rgs-containing sorting nexin controls drosophila lifespan一个rgs-containing排序nexin控制果蝇的寿命.pdfVIP

An RGS-Containing Sorting Nexin Controls Drosophila Lifespan 1 1 1 1 1 1,2,3 Jae Myoung Suh , Drew Stenesen , John M. Peters , Akiko Inoue , Angela Cade , Jonathan M. Graff * 1 Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 2 Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 3 Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America Abstract The pursuit of eternal youth has existed for centuries and recent data indicate that fat-storing tissues control lifespan. In a D. melanogaster fat body insertional mutagenic enhancer trap screen designed to isolate genes that control longevity, we identified a regulator of G protein signaling (RGS) domain containing sorting nexin, termed snazarus (sorting nexin lazarus, snz). Flies with insertions into the 59 UTR of snz live up to twice as long as controls. Transgenic expression of UAS-Snz from the snz Gal4 enhancer trap insertion, active in fat metabolic tissues, rescued lifespan extension. Further, the lifespan extension of snz mutants was independent of endosymbiont, e.g., Wolbachia, effects. Notably, old snz mutant flies remain active and fertile indicating that snz mutants have prolonged youthfulness, a goal of aging research. Since mammals have snz-related genes, it is possible that the functions of the snz family may be conserved to humans. Citation: Suh JM, Stenesen D, Peters JM, Inoue A, Cade A, et al. (2008) An RGS-Containing Sorting Nexin Controls Drosophila Lifespan. PLoS ONE 3(5): e2152. d