upscaled ctab-based dna extraction and real-time pcr assays for fusarium culmorum and f. graminearum dna in plant material with reduced sampling error高档ctab-based dna提取和实时pcr检测枯萎culmorum和graminearum dna在植物材料减少抽样误差.pdfVIP

下载本文档

10
0
约5.37万字
约 16页
2017-09-12 发布于上海
举报
版权申诉

upscaled ctab-based dna extraction and real-time pcr assays for fusarium culmorum and f. graminearum dna in plant material with reduced sampling error高档ctab-based dna提取和实时pcr检测枯萎culmorum和graminearum dna在植物材料减少抽样误差.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

upscaled ctab-based dna extraction and real-time pcr assays for fusarium culmorum and f. graminearum dna in plant material with reduced sampling error高档ctab-based dna提取和实时pcr检测枯萎culmorum和graminearum dna在植物材料减少抽样误差

Int. J. Mol. Sci. 2008, 9, 2306-2321; DOI: 10.3390/ijms9112306 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms/ Article Upscaled CTAB-Based DNA Extraction and Real-Time PCR Assays for Fusarium culmorum and F. graminearum DNA in Plant Material with Reduced Sampling Error Christoph Brandfass and Petr Karlovsky * University of Göttingen, Department of Crop Sciences, Molecular Phytopathology and Mycotoxin Research Division, Grisebachstrasse 6, 37077 Göttingen, Germany * Author to whom correspondence should be addressed; E-Mail: pkarlov@gwdg.de; Tel. +49-551-39-12918; Fax: +49-551-39-12919 Received: 28 August 2008; in revised form: 19 November 2008 / Accepted: 24 November 2008 / Published: 25 November 2008 Abstract: Fusarium graminearum Schwabe (Gibberella zeae Schwein. Petch.) and F. culmorum W.G. Smith are major mycotoxin producers in small-grain cereals afflicted with Fusarium head blight (FHB). Real-time PCR (qPCR) is the method of choice for species-specific, quantitative estimation of fungal biomass in plant tissue. We demonstrated that increasing the amount of plant material used for DNA extraction to 0.5-1.0 g considerably reduced sampling error and improved the reproducibility of DNA yield. The costs of DNA extraction at different scales and with different methods (commercial kits versus cetyltrimethylammonium