the first set of est resource for gene discovery and marker development in pigeonpea (cajanus cajan l.)第一组基因的est资源发现和标记发展木豆(cajanus毛竹和l .).pdfVIP

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the first set of est resource for gene discovery and marker development in pigeonpea (cajanus cajan l.)第一组基因的est资源发现和标记发展木豆(cajanus毛竹和l .).pdf

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the first set of est resource for gene discovery and marker development in pigeonpea (cajanus cajan l.)第一组基因的est资源发现和标记发展木豆(cajanus毛竹和l .)

Raju et al. BMC Plant Biology 2010, 10:45 /1471-2229/10/45 RESEARCH ARTICLE Open Access The first set of EST resource for gene discovery and marker development in pigeonpea (Cajanus cajan L.) 1 1,2 1 1 1 1 Nikku L Raju , Belaghihalli N Gnanesh , Pazhamala Lekha , Balaji Jayashree , Suresh Pande , Pavana J Hiremath , Munishamappa Byregowda2 3 1,4* , Nagendra K Singh , Rajeev K Varshney Abstract Background: Pigeonpea (Cajanus cajan (L.) Millsp) is one of the major grain legume crops of the tropics and subtropics, but biotic stresses [Fusarium wilt (FW), sterility mosaic disease (SMD), etc.] are serious challenges for sustainable crop production. Modern genomic tools such as molecular markers and candidate genes associated with resistance to these stresses offer the possibility of facilitating pigeonpea breeding for improving biotic stress resistance. Availability of limited genomic resources, however, is a serious bottleneck to undertake molecular breeding in pigeonpea to develop superior genotypes with enhanced resistance to above mentioned biotic stresses. With an objective of enhancing genomic resources in pigeonpea, this study reports generation and analysis of comprehensive resource of FW- and SMD- responsive expressed sequence tags (ESTs). Results: A total of 16 cDNA libraries were constructed from four pigeonpea genotypes that are resistant and susceptible to FW (’ICPL 20102’ and ‘ICP 2376’) and SMD (’ICP 7035’ and ‘TTB 7’) and a total of 9,888 (9,468 high quality) ESTs were generated and deposited in dbEST of GenBank under accession numbers GR463974 to GR473857 and GR958228 to GR958231.