the amt1 arginine methyltransferase gene is important for plant infection and normal hyphal growth in fusarium graminearumamt1精氨酸甲基转移酶基因对植物很重要感染和花药对赤霉病正常菌丝的生长.pdfVIP

The AMT1 Arginine Methyltransferase Gene Is Important for Plant Infection and Normal Hyphal Growth in Fusarium graminearum 1,2. 1. 1 2 2 1 Guanghui Wang , Chenfang Wang , Rui Hou , Xiaoying Zhou , Guotian Li , Shijie Zhang , Jin- Rong Xu2* 1 College of Plant Protection, Northwest AF University, Yangling, Shaanxi, China, 2 Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, United States of America Abstract Arginine methylation of non-histone proteins by protein arginine methyltransferase (PRMT) has been shown to be important for various biological processes from yeast to human. Although PRMT genes are well conserved in fungi, none of them have been functionally characterized in plant pathogenic ascomycetes. In this study, we identified and characterized all of the four predicted PRMT genes in Fusarium graminearum, the causal agent of Fusarium head blight of wheat and barley. Whereas deletion of the other three PRMT genes had no obvious phenotypes, the Damt1 mutant had pleiotropic defects. AMT1 is a predicted type I PRMT gene that is orthologous to HMT1 in Saccharomyces cerevisiae. The Damt1 mutant was slightly reduced in vegetative growth but normal in asexual and sexual reproduction. It had increased sensitivities to oxidative and membrane stresses. DON mycotoxin production and virulence on flowering wheat heads also were reduced in the Damt1 mutant. The introduction of the wild-type AMT1 allele fully complemented the defects of the Damt1 mutant and Amt1-GFP fusion proteins mainly localized to the nucleus. Hrp1 and Nab2 are two hnRNPs in yeast that are methylated by Hmt1 for nuclear export. In F. graminearum, AMT1 is requir