有哪些信誉好的足球投注网站temporal differences in microrna expression patterns in astrocytes and neurons after ischemic injury时间微rna表达模式的差异在缺血性损伤后星形胶质细胞和神经元.pdfVIP
- 1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
temporal differences in microrna expression patterns in astrocytes and neurons after ischemic injury时间微rna表达模式的差异在缺血性损伤后星形胶质细胞和神经元
Temporal Differences in MicroRNA Expression Patterns in Astrocytes and Neurons after Ischemic Injury Mateo Ziu, Lauren Fletcher, Shushan Rana, David F. Jimenez, Murat Digicaylioglu* Department of Neurosurgery, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America Abstract MicroRNAs (miRNAs) are small, non-protein-coding RNA molecules that modulate gene translation. Their expression is altered in many central nervous system (CNS) injuries suggesting a role in the cellular response to stress. Current studies in brain tissue have not yet described the cell-specific temporal miRNA expression patterns following ischemic injury. In this study, we analyzed the expression alterations of a set of miRNAs in neurons and astrocytes subjected to 60 minutes of ischemia and collected at different time-points following this injury. To mimic ischemic conditions and reperfusion in vitro, cortical primary neuronal and astrocytic cultures prepared from fetal rats were first placed in oxygen and glucose deprived (OGD) medium for 60 minutes, followed by their transfer into normoxic pre-conditioned medium. Total RNA was extracted at different time-points after the termination of the ischemic insult and the expression levels of miRNAs were measured. In neurons exposed to OGD, expression of miR-29b was upregulated 2-fold within 6 h and up to 4-fold at 24 h post-OGD, whereas induction of miR-21 was upregulated 2-fold after 24 h when compared to expression in neurons under normoxic conditions. In contrast, in astrocytes, miR-29b and miR-21 were upregulated only after 12 h. MiR-30b, 107, and 137 showed expression alteration in astrocytes, but not in neurons. Furthermore, we show that expression of miR-29b was significantly decreased in neurons exposed to Insulin-Like Growth Factor I (IGF-I), a well documented neuroprot
您可能关注的文档
- synthesis of the marine pyrroloiminoquinone alkaloids, discorhabdins海洋pyrroloiminoquinone生物碱的合成,discorhabdins.pdf
- synthesis of the marine bromotyrosine psammaplin f and crystal structure of a psammaplin a analogue合成海洋bromotyrosine psammaplin psammaplin f和晶体结构的模拟.pdf
- synthesis of the system modeling and signal detecting circuit of a novel vacuum microelectronic accelerometer合成系统的建模和信号检测电路的一种新型真空微电子加速度计.pdf
- synthesis of thiadiazoles and 1,2,4-triazoles derived from cyclopropane dicarboxylic acid合成thiadiazoles和1、2、4-triazoles来自环丙烷二羧酸.pdf
- synthesis of tetrasubstituted alkenes via metathesis通过交换tetrasubstituted烯烃的合成.pdf
- synthesis of triptorelin lactate catalyzed by lipase in organic media由脂肪酶在有机催化合成乳酸曲普瑞林的媒体.pdf
- synthesis of two new hemisynthetic diterpenylhydroquinones from natural ent-labdanes合成两个新的hemisynthetic diterpenylhydroquinones自然ent-labdanes.pdf
- synthesis of zinc oxide nanoparticles and their effect on the compressive strength and setting time of self-compacted concrete paste as cementitious composites氧化锌纳米颗粒的合成及其影响的抗压强度和凝结时间self-compacted混凝土粘贴胶结复合材料.pdf
- synthesis of silver nanoparticles in chitosan, gelatin and chitosangelatin bionanocomposites by a chemical reducing agent and their characterization合成银纳米粒子的壳聚糖、明胶和chitosangelatin bionanocomposites化学还原剂及其表征.pdf
- synthesis of xylooligosaccharides of daidzein and their anti-oxidant and anti-allergic activities合成xylooligosaccharides大豆苷及其抗氧化和抗敏的活动.pdf
- temporal dissection of k-rasg12d mutant in vitro and in vivo using a regulatable k-rasg12d mouse allele时间解剖k-rasg12d突变体在体外和体内使用regulatable k-rasg12d鼠标等位基因.pdf
- temporal objects—design, change and sustainability颞objects-design,变化和可持续性.pdf
- temporal dynamics of european bat lyssavirus type 1 and survival of myotis myotis bats in natural colonies时间动态的欧洲蝙蝠lyssavirus 1型和生存的鼠耳蝠鼠耳蝠蝙蝠在自然的殖民地.pdf
- temporal integration of movement the time-course of motion streaks revealed by masking时间集成运动运动条纹显示的时间进程屏蔽.pdf
- temporal and tissue specific regulation of rp-associated splicing factor genes prpf3, prpf31 and prpc8—implications in the pathogenesis of rp时间和组织特定的监管rp-associated剪接因子基因prpf3 prpf31和prpc8-implications rp的发病机理.pdf
- temporal pattern of icam-i mediated regulatory t cell recruitment to sites of inflammation in adoptive transfer model of multiple sclerosis时序模式icam-i调节性t细胞介导的炎症的招聘网站过继转移模型多发性硬化症.pdf
- temporal patterns in count-to-ten fetal movement charts and their associations with pregnancy characteristics a prospective cohort study时态模式数到十胎儿运动图表和他们对怀孕特征前瞻性队列研究.pdf
- temporal differential proteomes of clostridium difficile in the pig ileal-ligated loop model梭状芽胞杆菌的时序差异蛋白质组在猪ileal-ligated循环模型.pdf
- temporal network based analysis of cell specific vein graft transcriptome defines key pathways and hub genes in implantation injury时间基于网络分析的细胞特定静脉移植物转录组定义了关键路径和中心基因植入损伤.pdf
- temporal patterns of happiness and information in a global social network hedonometrics and twitter幸福的时间模式和信息在全球社交网络hedonometrics和twitter.pdf
文档评论（0）