telomeric trans-silencing in drosophila melanogaster tissue specificity, development and functional interactions between non-homologous telomeres端粒的trans-silencing在黑腹果蝇组织特异性、发展和功能异源染色体端粒之间的相互作用.pdfVIP

Telomeric Trans-Silencing in Drosophila melanogaster: Tissue Specificity, Development and Functional Interactions between Non-Homologous Telomeres Thibaut Josse1¤a, Corinne Maurel-Zaffran1¤b, Augustin de Vanssay1,2, Laure Teysset1,2, Anne-Laure 1,2 1,2 3 ´ ` 1 ´ Todeschini , Valerie Delmarre , Nicole Chaminade , Dominique Anxolabehere , Stephane Ronsseray1,2* ´ ´ 1 Laboratoire ‘‘Dynamique du Genome et Evolution’’, Institut Jacques Monod, UMR7592, CNRS-Universites Paris 6 et 7, Paris, France, 2 Laboratoire ‘‘Biologie du ´ ´ ´ ´ ˆ Developpement’’, UMR7622, CNRS-Universite Paris 6, Paris, France, 3 Laboratoire ‘‘Evolution, Genomes, Speciation’’, CNRS UPR 9034, Bat 13, Gif sur Yvette, France Abstract Background: The study of P element repression in Drosophila melanogaster led to the discovery of the telomeric Trans- Silencing Effect (TSE), a homology-dependent repression mechanism by which a P-transgene inserted in subtelomeric heterochromatin (Telomeric Associated Sequences, ‘‘TAS’’) has the capacity to repress in trans, in the female germline, a homologous P-lacZ transgene located in euchromatin. TSE can show variegation in ovaries, displays a maternal effect as well as an epigenetic transmission through meiosis and involves heterochromatin and RNA silencing pathways. Principal Findings: Here, we analyze phenotypic and genetic properties of TSE. We report that TSE does not occur in the soma at the adult stage, but appears restricted to the female g