striatal proteomic analysis suggests that first l-dopa dose equates to chronic exposure纹状体蛋白质组学分析表明,左旋多巴的剂量相当于慢性接触.pdfVIP

Striatal Proteomic Analysis Suggests that First L-Dopa Dose Equates to Chronic Exposure 1 1 1 ¨ 1 ¨ 1 1 ´ 2 Birger Scholz , Marcus Svensson , Henrik Alm , Karl Skold , Maria Falth , Kim Kultima , Celine Guigoni , 2 3 4 2. ´ 1. Evelyne Doudnikoff , Qin Li , Alan R. Crossman , Erwan Bezard *, Per E. Andren ´ 1 Department of Pharmaceutical Biosciences, Uppsala Biomedicinska Centrum (BMC), Uppsala University, Uppsala, Sweden, 2 Universite Victor Segalen Bordeaux 2, Centre National de la Recherche Scientifique, Bordeaux Institute of Neuroscience, UMR 5227, Bordeaux, France, 3 Institute of Lab Animal Sciences, China Academy of Medical Sciences, Beijing, China, 4 Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom Abstract L-3,4-dihydroxypheylalanine (L-dopa)-induced dyskinesia represent a debilitating complication of therapy for Parkinson’s disease (PD) that result from a progressive sensitization through repeated L-dopa exposures. The MPTP macaque model was used to study the proteome in dopamine-depleted striatum with and without subsequent acute and chronic L-dopa treatment using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The present data suggest that the dopamine-depleted striatum is so sensitive to de novo L-dopa treatment that the first ever administration alone would be able (i) to induce rapid post-translational modification-based proteomic changes tha