化痰降气胶囊对慢性阻塞性肺疾病治疗作用内在机理探究.docVIP

化痰降气胶囊对慢性阻塞性肺疾病治疗作用内在机理探究 　 作者：李泽庚， 张杰根， 彭波， 张念志， 韩明向 【关键词】 化痰降气胶囊；,,慢性阻塞性肺疾病；,,肿瘤坏死因子α；,,白三烯；,,大鼠 　　摘要：目的：探讨化痰降气胶囊对实验性慢性阻塞性肺疾病 治疗 作用的内在机理。 方法 ：采用经鼻腔注入肺炎克雷伯杆菌反复感染复制慢性阻塞性肺疾病模型大鼠，实验分为对照组、模型组和化痰降气胶囊组。并用酶联吸附实验(ELISA)方法，测定各组大鼠支气管灌洗液肿瘤坏死因子（TNFα）、白三烯（LTB4）水平。结果：模型组支气管灌洗液TNFα，LTB4水平较正常组显著升高(P＜0.01)；化痰降气胶囊组支气管灌洗液TNFα，LTB4水平均明显降低(P＜0.05)。结论：化痰降气胶囊可降低TNFα，LTB4的含量，控制慢性炎症反应。 毕业论文 　　关键词：化痰降气胶囊； 慢性阻塞性肺疾病； 肿瘤坏死因子α； 白三烯； 大鼠 毕业论文 　　Effect on the Level of TNFα, LTB4 in Bronchoalveolar Lavage Fluid of Model Rats with Chronic Obstructive Pulmonary Disease by Huatanjiangqi Capsule 毕业论文 　　　Abstract:Objective:To observe the effects of Huatanjiangqi Capsule, a combination of traditional Chinese medical herbs on the changes of TNFα,LTB4 in Bronchoalveolar Lavage Fluid, in order to provide basis for the explanation of the mechanisms of Huatanjiangqi Capsule and discover it’s pathophysiological characters.Methods:According to the principle of random, 36 Wistar rats(male)were divided into control group, model group and Huatanjiangqi Capsule group. model group was established by repeatedly intranasal infecting of klebsiella pneumoniae twice a week. TNFα，LTB4 in bronchoalvolar lavage fluid of three groups were measured by ELISA.Results:TNFα,LTB4 in bronchoalveolar lavage fluid of model group were significantly higher than in control group(P＜0.01), and the level of TNFα,LTB4 of Huatanjiangqi Capsule group were lower than those of the model group(P＜0.05).Conclusion:Huatanjiangqi Capsule could relieve the clinical symptoms and major injury of lung induced by superexcretion of inflame cytokine through dereasing the levels of TNFα,LTB4 in the rats with Chronic Obstructive Pulmonary Disease. 毕业论文 　　Key words:Huatanjiangqi Capsule; Chronic Obstructive Pulmonary Disease(COPD); Tumor Necrosis Factor alpha(TNFα); Leukotrienes B4(LTB4); Rats 　　化痰降气胶囊系经多年临床实践筛选出的治疗慢性支气管炎验方。 肿瘤坏死因子（TNFα）、白三烯（LTB4）是参与炎症反应的主要细胞因子之一，在慢性阻塞性肺疾病(COPD)的发病过程中起着关键作用［1］。为了进一步探讨化痰降气胶囊对慢性阻塞性肺疾病的作用机理，我们观察了其对慢性阻塞性肺疾病大鼠支气