【2017年整理】高效液相色谱法同时测定果蔬中多种防腐剂.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.6 高效液相色谱法同时测定果蔬中多种防腐剂 1 1 1 1 2 1 綦艳 ，黄秋研 ，梁德沛 ，周勇 ，樊垚 ，黄翠莉 （1.广东产品质量监督检验研究院，广东佛山 528300）（2.中南林业科技大学，湖南长沙 410004） 摘要：建立了高效液相色谱法同时测定水果及蔬菜中联苯、邻苯基苯酚、噻苯咪唑、抑霉唑、多菌灵、桂醛、乙萘酚、2, 4-二 氯苯氧乙酸的方法。样品用碱性乙醚提取，浓缩后甲醇定容，经ODS-SP C18（4.6×150 mm，5 µm）柱分离，以甲醇-磷酸盐缓冲液（pH=6.8 ） 为流动相，流速 0.8 mL/min 梯度洗脱，二极管阵列检测器（DAD）检测。此方法相关系数范围 0.9991~0.9997，加标回收率范围 88.0~97.4% ，相对标准偏差0.53~3.9% 。实验结果表明，该方法操作简单，结果灵敏准确，适用于水果及蔬菜中多种防腐剂的同时测 定。 关键词：高效液相色谱；果蔬；防腐剂 文章篇号：1673-9078(2013)6-1409-1412 Simultaneous Determination of Multiple Preservatives in Fruits and Vegetables by HPLC 1 1 1 1 2 1 QI Yan , HUANG Qiu-yan , LIANG De-pei , ZHOU Yong , FAN Yao , HUANG Cui-li (1.Guangdong Testing Institute for Product Quality Supervision, Foshan 528300, China) (2.Central south University of Forestry and Technology, Changsha 410004, China) Abstract: A HPLC method was developed for the determination of multiple preservatives in fruits and vegetables, including ortho-phenyl phenol, thiabendazole, imazalil, carbendazim, cinnamaldehyde, 2-naphthol, and 2,4-dichlorobenzene oxygen ethanoic acid. Samples were extracted by alkaline ether. The extract was dissolved in methanol and finally injected into the HPLC system after the extraction solution was evaporated to dry on a rotary evaporator. The separations were effected by the gradient elution mode using a mobile phase composed of methanol-phosphate buffer (pH 6.8) at the flow of 0.8 mL/min. The eight preservatives were separated on an ODS-SP C18 column and then detec