日本弓背蚁消化道厌氧细菌的分离培养-应用昆虫学报.PDFVIP

Chinese Journal of Applied Entomology 2015, 52(4): 10321039. DOI: 10.7679/j.issn.20951353.2015.124 * 日本弓背蚁消化道厌氧细菌的分离培养 ** *** 朱卓琳 南小宁 王云果 贺 虹 712100 摘 要 【目的】Camponotus japonicus 【方法】4 【结果】22 3 17 4 LB 10 8 MRS LBS 3 1 【结论】 关键词 The isolation of anaerobic bacteria from the gut of Camponotus japonicus ** *** ZHU Zhuo-Lin NAN Xiao-Ning WANG Yun-Guo HE Hong (College of Forestry, Northwest AF University, Yangling 712100, China) Abstract [Objectives] An anaerobic device was designed to culture anaerobic bacteria from the digestive tract of Camponotus japonicus. [Methods] A simple anaerobic device; the Interlayer Petri Dish Method, was improved to isolate anaerobic bacteria from the digestive tract of C. japonicus using four kinds of culture media (LB medium, Beef extract peptone, MRS medium and LBS medium). [Results] Twenty-two different bacterial strains were isolated from the guts of C. japonicus . These belonged to 17 genera of the Firmicutes, Actinobacteria and Proteobacterium. Four kinds of culture mediums had high selectivity, and isolated different bacterial species. More bacterial species were isolated from LB and beef extract peptone medium (10 and 8 species respectively), less were isolated fro