analysis of cells targeted by salmonella type iii secretion in vivo分析细胞的iii型分泌沙门氏菌体内的目标.pdfVIP
- 1、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
analysis of cells targeted by salmonella type iii secretion in vivo分析细胞的iii型分泌沙门氏菌体内的目标
Analysis of Cells Targeted by Salmonella
Type III Secretion In Vivo
1¤ 2 1*
Kaoru Geddes , Frank Cruz III , Fred Heffron
1 Department of Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, United States of America, 2 Department of Pathology, Oregon
Health and Science University, Portland, Oregon, United States of America
The type III secretion systems (TTSS) encoded in Salmonella pathogenicity island-1 and -2 (SPI-1 and -2) are virulence
factors required for specific phases of Salmonella infection in animal hosts. However, the host cell types targeted by
the TTSS have not been determined. To investigate this, we have constructed translational fusions between the ß-
lactamase reporter and a broad array of TTSS effectors secreted via SPI-1, SPI-2, or both. Secretion of the fusion protein
to a host cell was determined by cleavage of a specific fluorescent substrate. In cultured cells, secretion of all six
effectors could be observed. However, two to four days following i.p. infection of mice, only effectors secreted by SPI-2
were detected in spleen cells. The cells targeted were identified via staining with nine different cell surface markers
followed by FACS analysis as well as by conventional cytological methods. The targeted cells include B and T
lymphocytes, neutrophils, monocytes, and dendritic cells, but not mature macrophages. To further investigate
replication in these various cell types, Salmonella derivatives were constructed that express a red fluorescent protein.
Bacteria could be seen in each of the cell types above; however, most viable bacteria were present in neutrophils. We
find that Salmonella is capable of targeting most phagocytic and non-phagocytic cells in the spleen bu
您可能关注的文档
- amygdala engagement in response to subthreshold presentations of anxious face stimuli in adults with autism spectrum disorders preliminary insights杏仁核参与反应阈下演讲焦虑的脸刺激在成人自闭症谱系障碍的初步见解.pdf
- amyloid as a depot for the formulation of long-acting drugs淀粉样蛋白作为长效药物的配方的仓库.pdf
- amyloid precursor protein (app) mediated regulation of ganglioside homeostasis linking alzheimer's disease pathology with ganglioside metabolism淀粉样前体蛋白(app)介导的神经节苷脂调节体内平衡连接阿尔茨海默病病理和神经节苷脂代谢.pdf
- amplified genes may be overexpressed, unchanged, or downregulated in cervical cancer cell lines放大基因过表达,不变,或在宫颈癌细胞系表达下调.pdf
- amplification of a zygosaccharomyces bailii dna segment in wine yeast genomes by extrachromosomal circular dna formation放大的zygosaccharomyces bailii葡萄酒酵母基因组dna片段的染色体外的环状dna形成.pdf
- amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells淀粉样前体蛋白结合蛋白1在胎儿神经干细胞调节细胞周期进程.pdf
- amyloid-like fibril formation by polyq proteins a critical balance between the polyq length and the constraints imposed by the host proteinpolyq amyloid-like原纤维形成的蛋白质关键平衡polyq长度和宿主蛋白的限制.pdf
- amplified spontaneous emission properties of semiconducting organic materials有机半导体材料的放大自发发射性质.pdf
- amyloid-β oligomer specificity mediated by the igm isotype – implications for a specific protective mechanism exerted by endogenous auto-antibodiesamyloid-β低聚物特异性的igm同形像,对一个特定的内生auto-antibodies保护机制.pdf
- amyloid-mediated sequestration of essential proteins contributes to mutant huntingtin toxicity in yeastamyloid-mediated封存基本蛋白质有助于突变杭丁顿蛋白在酵母毒性.pdf
- an s-transform and support vector machine (svm)-based online method for diagnosing broken strands in transmission?liness-transform和支持向量机(svm)的在线诊断断链传输方法.pdf
- analysis of chaperone mrna expression in the adult mouse brain by meta analysis of the allen brain atlas分析成人伴侣蛋白mrna表达的小鼠大脑艾伦大脑图集的meta分析.pdf
- analysis of gene regulation in rabbit corneal epithelial cells induced by ultraviolet radiation分析基因调控在紫外线辐射引起的兔角膜上皮细胞.pdf
- analysis of endocrine disrupting pesticides by capillary gc with mass spectrometric detection内分泌扰乱杀虫剂通过毛细管gc分析质谱检测.pdf
- analysis of effective interconnectivity of degrapol-foams designed for negative pressure wound therapy分析有效互连degrapol-foams设计负压伤口治疗.pdf
- analysis of functional differences between hepatitis c virus ns5a of genotypes 1–7 in infectious cell culture systems功能上的差异分析丙型肝炎病毒基因型1 - 7的ns5a感染性细胞培养系统.pdf
- analysis of human accelerated dna regions using archaic hominin genomes分析人类加速使用陈旧的古人类基因组dna区域.pdf
- analysis of gene order conservation in eukaryotes identifies transcriptionally and functionally linked genes分析基因秩序的保护在真核生物识别转录和功能相关的基因.pdf
- analysis of immunoglobulin transcripts in the ostrich struthio camelus, a primitive avian species分析免疫球蛋白成绩单的鸵鸟struthio camelus,原始鸟类物种.pdf
- analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. what is in the plate分析人类和小鼠体细胞重编程的诱导多能干细胞。.pdf
最近下载
- 企业安全生产风险辨识评估管控指导手册-隧道工程施工.docx VIP
- 第二章口腔正畸工艺技术 .pptx VIP
- Aspen Hysys 扩展帮助手册.pdf VIP
- 普通高中数学课程标准(2017年版)学习资料.pdf VIP
- 创新型QC成果创新型QC成果.pdf VIP
- 2025年磷酸脲市场调研报告.docx VIP
- GB_T 43678-2024 生态系统评估 生态系统服务评估方法.pdf VIP
- 企业安全生产风险辨识评估管控指导手册-客运码头.docx VIP
- 【新教材】统编版(2019)高中政治必修1中国特色社会主义:第二课 只有社会主义才能救中国 共2课时教案.docx VIP
- 废气处理设备运行点检保养记录表.doc VIP
文档评论(0)