ambient ph controls glycogen levels by regulating glycogen synthase gene expression in neurospora crassa. new insights into the ph signaling pathway环境ph值控制糖原水平通过调节糖原合酶基因表达在粗糙脉孢菌。.pdfVIP

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ambient ph controls glycogen levels by regulating glycogen synthase gene expression in neurospora crassa. new insights into the ph signaling pathway环境ph值控制糖原水平通过调节糖原合酶基因表达在粗糙脉孢菌。.pdf

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ambient ph controls glycogen levels by regulating glycogen synthase gene expression in neurospora crassa. new insights into the ph signaling pathway环境ph值控制糖原水平通过调节糖原合酶基因表达在粗糙脉孢菌。

Ambient pH Controls Glycogen Levels by Regulating Glycogen Synthase Gene Expression in Neurospora crassa. New Insights into the pH Signaling Pathway 1 1 ˜ 2 Fernanda Barbosa Cupertino , Fernanda Zanolli Freitas , Renato Magalhaes de Paula , Maria ´ 1* Celia Bertolini ´ ´ ´ ˜ 1 Departamento de Bioquımica e Tecnologia Quımica, Instituto de Quımica, Universidade Estadual Paulista, UNESP, Araraquara, Sao Paulo, Brazil, 2 Nalco Company, Sugar Land, Texas, United States of America Abstract Glycogen is a polysaccharide widely distributed in microorganisms and animal cells and its metabolism is under intricate regulation. Its accumulation in a specific situation results from the balance between glycogen synthase and glycogen phosphorylase activities that control synthesis and degradation, respectively. These enzymes are highly regulated at transcriptional and post-translational levels. The existence of a DNA motif for the Aspergillus nidulans pH responsive transcription factor PacC in the promoter of the gene encoding glycogen synthase (gsn) in Neurospora crassa prompted us to investigate whether this transcription factor regulates glycogen accumulation. Transcription factors such as PacC in A. nidulans and Rim101p in Saccharomyces cerevisiae play a role in the signaling pathway that mediates adaptation to ambient pH by inducing the expression of alkaline genes and repressing acidic genes. We showed here that at pH 7.8 pacC was over- expressed and gsn was down-regulated in wild-type N. crassa coinciding with low glycogen accumulation. In