有哪些信誉好的足球投注网站an improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching一种改进的天蓝色荧光蛋白增强亮度和减少可逆的光电开关.pdfVIP
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an improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching一种改进的天蓝色荧光蛋白增强亮度和减少可逆的光电开关
An Improved Cerulean Fluorescent Protein with
Enhanced Brightness and Reduced Reversible
Photoswitching
1 2¤ 1 3 4
Michele L. Markwardt , Gert-Jan Kremers , Catherine A. Kraft , Krishanu Ray , Paula J. C. Cranfill ,
4 5 6 4 1
Korey A. Wilson , Richard N. Day , Rebekka M. Wachter , Michael W. Davidson , Mark A. Rizzo *
1 Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America, 2 Department of Molecular Physiology and
Biophysics, Vanderbilt University, Nashville, Tennessee, United States of America, 3 Center for Fluorescence Spectroscopy and the Department of Biochemistry and
Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America, 4 National High Magnetic Field Laboratory and Department
of Biological Science, The Florida State University, Tallahassee, Florida, United States of America, 5 Department of Cellular and Integrative Physiology, Indiana University
School of Medicine, Indianapolis, Indiana, United States of America, 6 Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona United States
of America
Abstract
¨
Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores in Forster resonance energy
transfer (FRET) experiments. Nonetheless, the most widely used variants suffer from drawbacks that include low quantum
yields and unstable flurorescenc
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