有哪些信誉好的足球投注网站an improved protocol for efficient engraftment in nodltsz-scidil-2rγnull mice allows hiv replication and development of anti-hiv immune responses一种改进的协议有效嫁接nodltsz-scidil-2rγnull老鼠让艾滋病毒复制和抗艾滋病病毒免疫反应的发展.pdfVIP
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an improved protocol for efficient engraftment in nodltsz-scidil-2rγnull mice allows hiv replication and development of anti-hiv immune responses一种改进的协议有效嫁接nodltsz-scidil-2rγnull老鼠让艾滋病毒复制和抗艾滋病病毒免疫反应的发展
An Improved Protocol for Efficient Engraftment in NOD/
LTSZ-SCIDIL-2RcNULL Mice Allows HIV Replication and
Development of Anti-HIV Immune Responses
1 1 1 1 2 3
Maneesh Singh , Pratibha Singh , Gilles Gaudray , Lucia Musumeci , Caroline Thielen , Dolores Vaira ,
1¤a 1 4 4 2¤b
Claire Vandergeeten , Laurence Delacroix , Ellen Van Gulck , Guido Vanham , Laurence de Leval ,
1 1
Souad Rahmouni , Michel Moutschen *
1 Immunology and infectious diseases unit GIGA-I3, University of Liege, Liege, Belgium, 2 Experimental pathology unit GIGA-Cancer, University of Liege, Liege, Belgium,
3 Liege AIDS reference center, University of Liege, Liege, Belgium, 4 Virology Unit Institute of Tropical Medicine, University of Antwerpen, Antwerpen, Antwerpen, Belgium
Abstract
Cord blood hematopoietic progenitor cells (CB-HPCs) transplanted immunodeficient NOD/LtsZ-scidIL2Rcnull (NSG) and
NOD/SCID/IL2Rcnull (NOG) mice need efficient human cell engraftment for long-term HIV-1 replication studies. Total body
irradiation (TBI) is a classical myeloablation regimen used to improve engraftment levels of human cells in these humanized
mice. Some recent reports suggest the use of busulfan as a myeloablation regimen to transplant HPCs in neonatal and adult
NSG mice. In the present study, we further ameliorated the busulfan myeloablation regimen with fresh CB-CD34+cell
transplantation in 3–4 week old NSG mice. In this CB-CD34+transplanted NSG mice engraftment efficiency of huma
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