adult neurogenesis ultrastructure of a neurogenic niche and neurovascular relationships成年神经发生神经源性小众的超微结构和神经与血管的关系.pdfVIP

Adult Neurogenesis: Ultrastructure of a Neurogenic Niche and Neurovascular Relationships 1,2 3 3 1,2 Paula Grazielle Chaves da Silva , Jeanne L. Benton , Barbara S. Beltz , Silvana Allodi * ´ ´ ˜ 1 Programa de Neurobiologia, Instituto de Biofısica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, 2 Programa de Pos-Graduac¸ao em ˆ ´ ˆ ´ Ciencias Morfologicas, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, 3 Neuroscience Program, Wellesley College, Wellesley, Massachusetts, United States of America Abstract The first-generation precursors producing adult-born neurons in the crayfish (Procambarus clarkii) brain reside in a specialized niche located on the ventral surface of the brain. In the present work, we have explored the organization and ultrastructure of this neurogenic niche, using light-level, confocal and electron microscopic approaches. Our goals were to define characteristics of the niche microenvironment, examine the morphological relationships between the niche and the vasculature and observe specializations at the boundary between the vascular cavity located centrally in the niche. Our results show that the niche is almost fully encapsulated by blood vessels, and that cells in the vasculature come into contact with the niche. This analysis also characterizes the ultrastructure of the cell types in the niche. The Type I niche cells are by