有哪些信誉好的足球投注网站a simple protocol for using a ldh-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time一个简单的协议使用ldh-based细胞毒性试验来评估死亡和生长抑制的影响在同一时间.pdfVIP
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a simple protocol for using a ldh-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time一个简单的协议使用ldh-based细胞毒性试验来评估死亡和生长抑制的影响在同一时间
A Simple Protocol for Using a LDH-Based Cytotoxicity
Assay to Assess the Effects of Death and Growth
Inhibition at the Same Time
1 2 1,3. 1 .
Shilo M. Smith *, Michael B. Wunder , David A. Norris , Yiqun G. Shellman *
1 Department of Dermatology, School of Medicine, University of Colorado Denver, Aurora, Colorado, United States of America, 2 Department of Integrative Biology,
University of Colorado, Denver, Colorado, United States of America, 3 Dermatology Section, Department of Veterans Affairs Medical Center, Denver, Colorado, United
States of America
Abstract
Analyzing the effects on cell growth inhibition and/or cell death has been an important component of biological research.
The MTS assay and LDH-based cytotoxicity assays are two of the most commonly used methods for this purpose. However,
data here showed that MTS cell proliferation assay could not distinguish the effects of cell death or cell growth inhibition. In
addition, the original LDH-based cytotoxicity protocol grossly underestimated the proportion of dead cells in conditions
with growth inhibition. To overcome the limitation, we present here a simple modified LDH-based cytotoxicity protocol by
adding additional condition-specific controls. This modified protocol thus can provide more accurate measurement of
killing effects in addition to the measurement of overall effects, especially in conditions with growth inhibition. In summary,
we present here a simple, modified cytotoxicity assay, which can determine the overall effects, percentage of cell killing and
growth inhibition in one 96-well based assay. This is a viable option for primary screening for many laboratories, and could
be adapted for high throughput screening.
Citation: Smith SM,
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