a simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers一个简单bioconjugate附件协议使用武力在单分子光谱实验基于混合自组装单层膜.pdfVIP

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a simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers一个简单bioconjugate附件协议使用武力在单分子光谱实验基于混合自组装单层膜.pdf

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a simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers一个简单bioconjugate附件协议使用武力在单分子光谱实验基于混合自组装单层膜

Int. J. Mol. Sci. 2012, 13, 13521-13541; doi:10.3390/ijms131013521 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A Simple Bioconjugate Attachment Protocol for Use in Single Molecule Force Spectroscopy Experiments Based on Mixed Self-Assembled Monolayers Simon J. Attwood 1, Anna M. C. Simpson 2, Rachael Stone 2, Samir W. Hamaia 2, Debdulal Roy 3, Richard W. Farndale 2, Myriam Ouberai 1 and Mark E. Welland 1* 1 Nanoscience Centre, Department of Engineering, Cambridge University, Cambridge, CB3 0FF, UK; E-Mails: simonjamesattwood@ (S.J.A.); mmo25@cam.ac.uk (M.O.) 2 Department of Biochemistry, Cambridge University, Cambridge, CB2 1QW, UK; E-Mails: anna.simpson@ (A.M.C.S.); rj271@mole.bio.cam.ac.uk (R.S.); swh23@cam.ac.uk (S.W.H.); rwf10@cam.ac.uk (R.W.F.) 3 National Physical Laboratory, Teddington, TW11 0LW, UK; E-Mail: debdulal.roy@npl.co.uk * Author to whom correspondence should be addressed; E-Mail: mew10@eng.cam.ac.uk; Tel.: +44-1223-760305; Fax: +44 -1223-760309. Received: 22 August 2012; in revised form: 9 September 2012 / Accepted: 29 September 2012 / Published: 19 October 2012 Abstract: Single molecule force spectroscopy is a technique that can be used to probe the interaction force between individual biomolecular species. We focus our attention on the tip and sample coupling chemistry, which is crucial to these experiments. We utilised a novel approach of mixed self-assembled monolayers of alkanethiols in conjunc