有哪些信誉好的足球投注网站a rapid and highly sensitive method of non radioactive colorimetric in situ hybridization for the detection of mrna on tissue sections快速和高度敏感的非放射性的方法比色检测mrna的原位杂交组织部分.pdfVIP
- 1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a rapid and highly sensitive method of non radioactive colorimetric in situ hybridization for the detection of mrna on tissue sections快速和高度敏感的非放射性的方法比色检测mrna的原位杂交组织部分
A Rapid and Highly Sensitive Method of Non Radioactive
Colorimetric In Situ Hybridization for the Detection of
mRNA on Tissue Sections
1. 1. 2 2
Electra Stylianopoulou , Dimitrios Lykidis , Petros Ypsilantis , Constantinos Simopoulos ,
1 1
George Skavdis , Maria Grigoriou *
1 Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece, 2 School of Medicine, Democritus University of Thrace,
Alexandroupolis, Greece
Abstract
Background: Non Radioactive colorimetric In Situ Hybridization (NoRISH) with hapten labeled probes has been widely used
for the study of gene expression in development, homeostasis and disease. However, improvement in the sensitivity of the
method is still needed to allow for the analysis of genes expressed at low levels.
Methodology/Principal Findings: A stable, non-toxic, zinc-based fixative was tested in NoRISH experiments on sections of
mouse embryos using four probes (Lhx6, Lhx7, ncapg and ret) that have different spatial patterns and expression levels. We
showed that Z7 can successfully replace paraformaldehyde used so far for tissue fixation in NoRISH; the morphology of the
cryosections of Z7-fixed tissues was excellent, and the fixation time required for tissues sized 1 cm was 1 hr instead of 24 hr
for paraformaldehyde. The hybridization signal on the sections of the Z7-treated embryos always appeared earlier than that
of the PFA-fixed embryos. In addition, a 50–60% shorter detection time was observed in specimen of Z7-treated embryos,
reducing significantly the time required to complete the method. Finally and most importantly, the strength of the
hybridization signal on th
您可能关注的文档
- a novel endogenous inhibitor of the secreted streptococcal nad-glycohydrolase一种新型链球菌nad-glycohydrolase分泌的内源性抑制剂.pdf
- a novel cyclodextrin glycosyltransferase from alkaliphilic amphibacillus sp. npst-10 purification and properties一种新型环糊精糖基转移酶从alkaliphilic amphibacillus sp。npst-10净化和属性.pdf
- a novel cold-adapted lipase from sorangium cellulosum strain so0157-2 gene cloning, expression, and enzymatic characterization一种新型低温脂肪酶从sorangium cellulosum应变so0157-2基因克隆,表达和酶的特性.pdf
- a novel dynamic impact approach (dia) for functional analysis of time-course omics studies validation using the bovine mammary transcriptome一种新颖的动态影响方法(dia)功能分析的时间进程组学研究验证使用牛乳腺转录组.pdf
- a novel enzymatic system against oxidative stress in the thermophilic hydrogen-oxidizing bacterium hydrogenobacter thermophilus一种新型酶系统对氧化应激的嗜热细菌种氢氧hydrogenobacter酸奶.pdf
- a novel extracellular hsp90 mediated co-receptor function for lrp1 regulates epha2 dependent glioblastoma cell invasion一种新的细胞外一半介导的受体功能lrp1恰巧调节epha2依赖胶质母细胞瘤细胞的入侵.pdf
- a novel estimator for the rate of information transfer by continuous signals一种新颖的估计量的信息传递的速度连续信号.pdf
- a novel fractional order model for the dynamic hysteresis of piezoelectrically actuated fast tool servo一种新颖的分数阶模型的动态滞后piezoelectrically伺服驱动快速工具.pdf
- a novel chemometric method for the prediction of human oral bioavailability一种新型最优化方法预测人类的口服生物利用度.pdf
- a novel generalized normal distribution for human longevity and other negatively skewed data一种新的广义正态分布对人类寿命和其他负面倾斜的数据.pdf
- a quantitative, non-destructive methodology for habitat characterisation and benthic monitoring at offshore renewable energy developments量化,非破坏性方法栖息地的描述和底栖生物监测海洋可再生能源的发展.pdf
- a rapid change in virulence gene expression during the transition from the intestinal lumen into tissue promotes systemic dissemination of salmonella迅速改变肠道流明的毒性基因表达在过渡到组织中促进系统性传播沙门氏菌.pdf
- a rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimens一个快速和简单的程序建立人类正常和癌症手术标本的肾原代细胞培养.pdf
- a rapid method for optimizing running temperature of electrophoresis through repetitive on-chip ce operations优化运行温度的快速方法通过重复的片上电泳ce操作.pdf
- a rapid crosstalk of human γδ t cells and monocytes drives the acute inflammation in bacterial infections人类γδt细胞和单核细胞的快速相声驱动器细菌感染的急性炎症.pdf
- a rapid sound-action association effect in human insular cortex在人类岛叶皮质快速行为关联效应.pdf
- a rapid flp-in system for expression of secreted h5n1 influenza hemagglutinin vaccine immunogen in mammalian cells快速flp-in分泌h5n1流感病毒血凝素的表达系统在哺乳动物细胞疫苗免疫原.pdf
- a realistic validation study of a new nitrogen multiple-breath washout system一个现实的验证研究一个新的氮multiple-breath冲洗系统.pdf
- a rapid subtractive immunization method to prepare discriminatory monoclonal antibodies for food e. coli o157h7 contamination快速减去免疫方法准备歧视性的单克隆抗体对大肠杆菌o157h7污染食物.pdf
- a real-time pcr antibiogram for drug-resistant sepsis实时pcr为耐药败血症抗菌谱.pdf
文档评论（0）