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a rapid method for optimizing running temperature of electrophoresis through repetitive on-chip ce operations优化运行温度的快速方法通过重复的片上电泳ce操作
Int. J. Mol. Sci. 2011, 12, 4271-4281; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
A Rapid Method for Optimizing Running Temperature of
Electrophoresis through Repetitive On-Chip CE Operations
Shohei Kaneda 1,2, Koichi Ono 3,4, Tatsuhiro Fukuba 5, Takahiko Nojima 6, Takatoki Yamamoto 7
and Teruo Fujii 1,2,*
1 LIMMS-CNRS/IIS (UMI2820), Institute of Industrial Science, University of Tokyo,
Tokyo 153-8505, Japan; E-Mail: shk@iis.u-tokyo.ac.jp
2 JST CREST, Tokyo 102-0075, Japan
3 Enplas Corporation, Saitama 332-0034, Japan; E-Mail: k-ono@iis.u-tokyo.ac.jp
4 Center for International Research on Micronano Mechatronics, Institute of Industrial Science,
University of Tokyo, Tokyo 153-8505, Japan
5 Ocean Alliance, University of Tokyo, Chiba 277-8564, Japan; E-Mail: bafuk@iis.u-tokyo.ac.jp
6 College of Liberal Arts and Sciences, Kitasato University, Kanagawa 252-0373, Japan;
E-Mail: nojima@kitasato-u.ac.jp
7 Department of Mechano-Aerospace Engineering, Tokyo Institute of Technology, Tokyo 152-8550,
Japan; E-Mail: yamamoto@mes.titech.ac.jp
* Author to whom correspondence should be addressed; E-Mail: tfujii@iis.u-tokyo.ac.jp;
Tel.: +81-3-5452-6211; Fax: +81-3-5452-6212.
Received: 6 April 2011; in revised form: 16 June 2011 / Accepted: 20 June 2011 /
Published: 1 July 2011
Abstract: In this paper, a rapid and simple method to determine the optimal temperature
conditions for denaturant electrophoresis using
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