a portable chemotaxis platform for short and long term analysis一个便携式趋化作用短期和长期分析的平台.pdfVIP

A Portable Chemotaxis Platform for Short and Long Term Analysis 1,3 1 1 1 1 1 Chenjie Xu , Yuk Kee C. Poh , Isaac Roes , Eoin D. O’Cearbhaill , Mads Emil Matthiesen , Luye Mu , 1 1 2 1 Seung Yun Yang , David Miranda-Nieves , Daniel Irimia , Jeffrey M. Karp * 1 Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women’s Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT, Division of Health Sciences and Technology, Cambridge, Massachusetts, United States of America, 2 BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Shriners Hospital for Children, and Harvard Medical School, Boston, Massachusetts, United States of America, 3 Division of Bioengineering, School of Chemical Biomedical Engineering, Nanyang Technological University, Singapore Abstract Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZETH osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate an