a new highly conserved antibiotic sensingresistance pathway in firmicutes involves an abc transporter interplaying with a signal transduction system一种新的高度保守的抗生素sensingresistance通路在厚壁菌门包括abc转运蛋白相互作用的信号转导系统.pdfVIP

A New Highly Conserved Antibiotic Sensing/Resistance Pathway in Firmicutes Involves an ABC Transporter Interplaying with a Signal Transduction System ´ 1,2 ´ 1,3 1,2 1,2 Stephanie Coumes-Florens , Celine Brochier-Armanet , Annick Guiseppi , Franc¸ois Denizot *, Maryline Foglino1,2 ´ ´ ´ ´ ´ ´ 1 Laboratoire de Chimie Bacterienne (UPR9043), Institut de Microbiologie de la Mediterranee (IFR88), CNRS, Marseille, France, 2 Universite de la Mediterranee, Marseille, ´ France, 3 Universite de Provence, Marseille, France Abstract Signal transduction systems and ABC transporters often contribute jointly to adaptive bacterial responses to environmental changes. In Bacillus subtilis, three such pairs are involved in responses to antibiotics: BceRSAB, YvcPQRS and YxdJKLM. They are characterized by a histidine kinase belonging to the intramembrane sensing kinase family and by a translocator possessing an unusually large extracytoplasmic loop. It was established here using a phylogenomic approach that systems of this kind are specific but widespread in Firmicutes, where they originated. The present phylogenetic analyses brought to light a highly dynamic evolutionary history involving numerous horizontal gene transfers, duplications and lost events, leading to a great variety of Bce-like repertories in members of this bacterial phylum. Based on these phylogenetic analyses, it was proposed to subdivide the Bce-like modules into six well-defined subfamilies. Functional studies were performed on mem