a new strategy for identification of highly conserved micrornas in non-model insect, spodoptera litura高度保守的小分子核糖核酸的新战略识别non-model昆虫,spodoptera litura.pdfVIP

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a new strategy for identification of highly conserved micrornas in non-model insect, spodoptera litura高度保守的小分子核糖核酸的新战略识别non-model昆虫,spodoptera litura.pdf

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a new strategy for identification of highly conserved micrornas in non-model insect, spodoptera litura高度保守的小分子核糖核酸的新战略识别non-model昆虫,spodoptera litura

Int. J. Mol. Sci. 2012, 13, 612-627; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A New Strategy for Identification of Highly Conserved microRNAs in Non-Model Insect, Spodoptera litura Lu Gao, Hongliang Zuo, Keling Liu, Haiyi Li and Guohua Zhong * Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China; E-Mails: gaolu0424@163.com (L.G.); zhlzuo@ 163.com (H.Z.); liuwkwl@126.com (K.L.); oceanyili@163.com (H.L.) * Author to whom correspondence should be addressed; E-Mail: guohuazhong@; Tel.: +86-20-8528-0308; Fax: +86-20-8528-0292. Received: 19 October 2011; in revised form: 23 November 2011 / Accepted: 28 December 2011 / Published: 9 January 2012 Abstract: The indigenous small non-coding RNAs, known as microRNAs (miRNAs), are important regulators of gene expression and many of them are evolutionarily conserved. Whether stem-loop RT-PCR, as a sensitive method, could be utilized to clone conserved miRNAs from non-model insects lacks information. Here, three miRNAs, sli-miR-14, sli-miR-2a and sli-bantam, were cloned from Spodoptera litura by stem-loop RT-PCR. Two groups of primers were designed, and one of them performed especially well and proved stable. The sequences of two highly conserved miRNAs, sli-miR-14 and sli-miR-2a were identical to those