有哪些信誉好的足球投注网站a genome-wide survey of switchgrass genome structure and organization全基因组调查柳枝稷基因组的结构和组织.pdfVIP
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a genome-wide survey of switchgrass genome structure and organization全基因组调查柳枝稷基因组的结构和组织
A Genome-Wide Survey of Switchgrass Genome
Structure and Organization
1,2 1,2 3 4,5 1,2¤ 4,5
Manoj K. Sharma , Rita Sharma , Peijian Cao , Jerry Jenkins , Laura E. Bartley , Morgan Qualls ,
Jane Grimwood4,5, Jeremy Schmutz4,5, Daniel Rokhsar5,6, Pamela C. Ronald1,2*
1 Department of Plant Pathology, University of California Davis, Davis, California, United States of America, 2 Joint BioEnergy Institute, Emeryville, California, United States
of America, 3 China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute, Zhengzhou, China, 4 HudsonAlpha Institute of Biotechnology, Huntsville,
Alabama, United States of America, 5 United States Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America, 6 University of
California, Berkeley, California, United States of America
Abstract
The perennial grass, switchgrass (Panicum virgatum L.), is a promising bioenergy crop and the target of whole genome
sequencing. We constructed two bacterial artificial chromosome (BAC) libraries from the AP13 clone of switchgrass to gain
insight into the genome structure and organization, initiate functional and comparative genomic studies, and assist with
genome assembly. Together representing 16 haploid genome equivalents of switchgrass, each library comprises 101,376
clones with average insert sizes of 144 (HindIII-generated) and 110 kb (BstYI-generated). A total of 330,297 high quality BAC-
end sequences (BES) were generated, accounting for 263.2 Mbp (16.4%) of the switchgrass genome. Analysis of the BES
identified 279,099 known repetitive elements, .50,000 SSRs, and 2,528 novel repeat elements, named switchgrass
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