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a bac-based physical map of zhikong scallop (chlamys farreri jones et preston)bac-based物理地图zhikong扇贝(斗篷farreri琼斯et preston)
A BAC-Based Physical Map of Zhikong Scallop (Chlamys
farreri Jones et Preston)
1 1,2 1,2 1,2 1 1 1,2
Xiaojun Zhang , Cui Zhao , Chao Huang , Hu Duan , Pin Huan , Chengzhang Liu , Xiuying Zhang ,
3 1 3 1
Yang Zhang , Fuhua Li , Hong-Bin Zhang , Jianhai Xiang *
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China, 2 Graduate University of the Chinese Academy
of Sciences, Beijing, China, 3 Department of Soil and Crop Sciences, Texas AM University, College Station, Texas, United States of America
Abstract
Zhikong scallop (Chlamys farreri) is one of the most economically important aquaculture species in China. Physical maps are
crucial tools for genome sequencing, gene mapping and cloning, genetic improvement and selective breeding. In this
study, we have developed a genome-wide, BAC-based physical map for the species. A total of 81,408 clones from two BAC
libraries of the scallop were fingerprinted using an ABI 3130xl Genetic Analyzer and a fingerprinting kit developed in our
laboratory. After data processing, 63,641 (,5.8 6genome coverage) fingerprints were validated and used in the physical
map assembly. A total of 3,696 contigs were assembled for the physical map. Each contig contained an average of 10.0
clones, with an average physical size of 490 kb. The combined total physical size of all contigs was 1.81 Gb, equivalent to
approximately 1.5 fold of the scallop haploid genome. A total of 10,587 BAC end sequences (BESs) and 167 markers were
integrated into the physical map. We evaluated the physical map by overgo hybridization, B
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