a bac-based transgenic mouse specifically expresses an inducible cre in the urotheliumbac-based转基因老鼠特别表达的诱导cre尿道上皮.pdfVIP

A BAC-Based Transgenic Mouse Specifically Expresses an Inducible Cre in the Urothelium Tian Huai Shen1,2¤a, Nataliya Gladoun1,2¤b, Mireia Castillo-Martin1,2,3¤b, Dennis Bonal1,2,3¤b, 1,2,3¤b 1 1,2,3¤b Josep Domingo-Domenech , Daniel Charytonowicz , Carlos Cordon-Cardo * 1 Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America, 2 Department of Pathology, Columbia University Medical Center, New York, New York, United States of America, 3 Department of Urology, Columbia University Medical Center, New York, New York, United States of America Abstract Cre-loxp mediated conditional knockout strategy has played critical roles for revealing functions of many genes essential for development, as well as the causal relationships between gene mutations and diseases in the postnatal adult mice. One key factor of this strategy is the availability of mice with tissue- or cell type-specific Cre expression. However, the success of the traditional molecular cloning approach to generate mice with tissue specific Cre expression often depends on luck. Here we provide a better alternative by using bacterial artificial chromosome (BAC)-based recombineering to insert iCreERT2 cDNA at the ATG start of the Upk2 gene. The BAC-based transgenic mice express the inducible Cre specifically in the urothelium as demonstrated by mRNA expression and staining for LacZ expression after crossing with a Rosa26 reporter mouse. Taking into consideration the size of the gene of interest and neighboring genes included in a BAC, this method should be widely applicable for generation of mice with tissue specific gene expression or deletions in a more specific