外周血游离DNA KRAS基因突变检测方法的建立及临床应用.pdfVIP

目录 中文 摘 要 ·····························································································，···········……1 英 文 摘 要 ·········································································································……3 论 文 正 文 前 言 ·························································································，·······················……5 一 、高 敏 感 H R 入1 法 检 测 犬凡理S 基 因突 变 方 法 的建 立 引言 ·················································································································……8 材 料 和 方 法 ···································，·································································……9 结 果 ···············································································································……16 讨 论 ····················，···························································，······························……21 二 、非标 一记探 针 一H RM 法 检 测 犬天且S 基 因突变 在 胰 腺 癌 组 织 中 的应 用 引言 ·······················································，·······················································……24 材 料 和 方 法 ···································································，·······························……25 结 果 ···············································································································……33 讨 论 ····，······································································，···································……34 三 、C O L D 一P C R 法 检 测 尤凡搜S 基 因突变 在 胰 腺癌 患者 外周 血 中的应 用 引 言 ·······························································，·······································，·······，·…36 材 料 和 方 法 ···································································································……37 结 果 ·····························································，························，························……40 讨 论 ···············································································································……42 小 结 ······