Chordoma cells in vitro invasiveness（脊索瘤细胞体外侵袭性）.doc

Chordoma cells in vitro invasiveness Author: Chang Heng Ma Baoan Zhangdian Zhong Wen Yanhua [Keywords] chordoma Keywords: chordoma; tumor invasion; urokinase-type plasminogen activator; immunohistochemistry Abstract: Objective To observe the chordoma cells in vitro invasiveness of size, explore closely related with tumor aggressiveness urokinase-type plasminogen activator (uroki-nase-type plasminogen activator uPA in the invasion. Methods chordoma cells in vitro invasion model of the sphere observed invasion characteristics and invasion of tumor spheroids; detected by immunohistochemical SABC method the chordoma cell expression of uPA and observed with uPA antibody invasive tumor cells change in chick embryo myocardial block (preculture heart fragment, PHF) contact preculture 3d surrounding PHF, and part of the invasion, contact training 5d, each PHF that about half of invasion, the 10d left and right, PHF completely displaced by the invasion. immunohistochemical staining, positive cells accounted for 63% of the high expression of uPA., tumor cell invasiveness reduce uPA antibody added to the culture medium. conclusion chordoma cells with certain invasive; uPA high expression of the cell; uPA antibody The chordoma cells can be invasive reduce. Keywords: chordoma; invasion; urokinase-type plasminogen activator; immunohistochemistry Abstract: AIM To evaluate the invasion of human chordo-ma cell in vitro, and the effects of urokinase-type plasmino-gen activator on tumour invasion.METHODS The invasive capacity and the invasive process of chordoma cells were ob-served with invasion model of tumour cell spheroid aggregate in vitro; Applying streptavidin-biotin complex (SABCimmunohistochemical technique, the expression of uPA in human chordoma cell line was studied.RESULTS 3d after PHFs confronted cultured with tumour cell spheroid aggre-gates, they were sphered and invaded.5d after confronting cultured, about the half of PHF was invaded and10days af-ter,