珍珠梅黄酮纳米粒抑制脂多糖诱发肝癌细胞炎性作用的研究-药学学报.PDFVIP

珠梅黄酮纳米粒抑制脂多糖诱发肝癌细胞炎性作用的研究1 邵葵阳，张璇，焦文君，张斯琳，张学武* （延边大学医学院，吉林延吉 133002） 摘要：探讨珍珠梅黄酮纳米粒（5,2 ,4 -trihydroxy-6,7,5 -trimethoxy flavone nanoparticle ，TTF 1-NP ）抑制脂多糖（lipopoiysaccharide，LPS ）诱发人肝癌HepG2 细胞的炎性作用及调控机制。采用LPS 诱发人肝癌HepG2 细胞的炎症模型；MTT 比色法检测 TTF 1-NP 对人肝癌 HepG2 细胞增殖作用；Western blot 法检测 TTF 1-NP 对LPS 诱发的HepG2 细胞炎性反应的TLR4 和IL-6 及AKT/mTOR 信 号通路关键蛋白的表达作用。结果显示，TTF1-NP 能够抑制LPS 引起的人肝癌 HepG2 细胞增殖，抑制TLR4 蛋白表达及AKT 和mTOR 蛋白的磷酸化，抑制炎 症因子IL-6 和IL-8 表达；TTF 1-NP 抑制insulin 引起的AKT/mTOR 信号通路的 活化和TLR4 蛋白的表达，从而抑制IL-6 和IL-8 蛋白表达。研究表明，TTF 1-NP 抑制LPS 诱导的人肝癌HepG2 细胞的炎性反应，其作用机制可能与AKT/mTOR 信号通路有关。 关键词：珍珠梅黄酮纳米粒；肝癌；炎症；蛋白激酶 B/哺乳动物雷帕霉素 靶蛋白信号通路 中图分类号：R965 文献标识码：A 文章编号：0513-4870(2017) The anti-inflammatory effect of TTF1-NP on lipopoiysaccharide stimulation human hepatocellular carcinoma cells SHAO Kui-yang, ZHANG Xuan, JIAO Wen-jun, ZHANG Si-lin, ZHANG Xue-wu* (Medical College, Yanbian University, Yanji 133002, China) Abstract: The study is designed to discuss the role of 5,2 ,4 -trihydroxy-6,7,5 -trimethoxy flavone nanoparticle (TTF 1-NP) on 收稿日期：20 17-05-03 修回日期：20 17-06-21. 基金项目：国家自然科学基金资助项目 . *通讯作者Tel: 86-433-2435102, Fax: 86-433-2435104, E-mail: zhangxuewu@ybu.edu.cn DOI: 10.16438/j.0513-4870.2017-0432 lipopoiysaccharide (LPS)-induced inflammatory response, and its anti-inflammatory mechanism in human hepatocellular carcinoma cells. Inflammatory responses were induced by LPS in human hepatocellular carcinoma HepG2 cells; Proliferation effect of TTF 1-NP in LPS-stimulated HepG2 cells were detected by MTT assay; The expression of TLR4, AKT/mTOR signaling related proteins and IL-6 were detected by Western blot assay. The results showed that TTF 1-NP inhibited the proliferation of HepG2 cells induced by LPS in a dose-dependent mann