碳水化合物分析柱使用手册（Carbohydrate analysis column manual）.docVIP

碳水化合物分析柱使用手册（Carbohydrate analysis column manual） Carbohydrate analysis column manual (2008-06-02 19:14:16) reprint we label: water compounds on classification of carbon: professional - chromatography analysis Be careful The Chrompack Carbohydrates Ca column is filled with polymer material that requires special maintenance. Introducing an organic solvent other than the introduction to the column results in expansion and over pressurization of the polymer material. Therefore, you should use isopropanol before you install the column, and then rinse the pipe thoroughly with deionized water to completely remove the organic solvent. Before using the column, be fully familiar with what the manual tells you. 1. introduction The Chrompack Carbohydrates Ca column contains a cationic removal resin of Ca++ morphology, specifically designed for single chromatographic separation of polysaccharides, mono oligosaccharides and sugar alcohols. 2. elution Only recommend the use of deionized water (especially for removing metal ions and halogen compounds), and sterile (through a 0.45 micron filter). Water of grade HPLC can be bought and used on this column. It works well. The eluate is degassed before being used and stored in a container to prevent airborne contamination of bacteria and fungi. Fresh mobile phases should be prepared every 24 hours. Never use methanol, tetrahydrofuran, DMF, organic or inorganic acids, strong bases on this column. Once one of the above solvents is used for this column, use 11.3 of the polymer cleaning step for maintenance. Injection of high concentrations of cations can cause irreversible damage to the column. 3. flow and pressure To limit elution flow, the pressure must not exceed 1500PSI. The recommended flow rate for the Chrompack Carbohydrates Ca column is.5ml/min. The maximum traffic possible is probably 0.7 ml/min. Remember the required drive, when the eluent flows through the column, the pump pressure is caused by elution, flow rate, column temper