现代液相色谱进展(二)（Advances in modern liquid chromatography (two)）.docVIP

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现代液相色谱进展(二)（Advances in modern liquid chromatography (two)）.doc

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现代液相色谱进展(二)（Advances in modern liquid chromatography (two)）

现代液相色谱进展(二)（Advances in modern liquid chromatography (two)） The development of modern liquid chromatography (two.Txt30) the beauty of life, never to show her ahead; as beautiful trees, is displayed in its negative potential to touch the sky in the vigor; as beautiful eagle, is displayed in its fight to fly the wind and the rain as God in the soul of the beautiful like rivers;, is displayed in its roaring waves flowing torrent in. 2, liquid chromatography, mobile phase Reverse phase chromatography is widely used in liquid chromatography. The optimization of its mobile phase is directly related to the separation effect. In 80s, the optimization of the composition of the mobile phase of two yuan depended on the relationship between the numerical value of the capacity factor (k) and the molar fraction of polar solvent, and the retention value of the solute could be estimated according to the linear relationship between them. The relation between the capacity factor of 14 kinds of two solvent system and the mobile phase has been determined. Scott, Snyder and Soczeuinski chromatography model is applied to the separation of alkyl phenol and naphthol, using mBondapakNH2 stationary phase, Nurok and Richard developed a simple equation to determine the optimal solvent composition, this method can be used for TLC can also be used for HPLC. The combination of six plasma strength solvents is applied to produce six three element mobile phases for separating large amounts of compounds whose polarity is modulated by increasing polarity of the solvent. Zima and Smolkova reported the acid-base effect and ionic strength of the amino acid retention values on the carbon adsorbent, and gave a series of equations predicting the retention behavior of these electrolytes. Markl has added organophosphorus and sulfur to the mobile phase, which can adjust the surface of silica gel. These compounds are adsorbed on the surface of silica gel and change the selectivity and solute retention value bec