现代生物学实验习题（Modern biology experiment exercises）.docVIP

现代生物学实验习题（Modern biology experiment exercises） Documents from the network, I collected, finishing, and if there are omissions, errors, but also please correct me! Modern biology experiment exercises (1) each experiment is completed in accordance with the format of experimental report paper (two) answer the questions in each module of each experiment Thinking summary: Part 1: biochemistry experiment The first module: sugar experimental technology 1, how to select the experimental materials in biochemical substances experiment? What are the criteria? 2, what are the materials crushing methods? 3. Alkaline copper reagent method is the direct or indirect titration of reducing sugar? What are the advantages of the two titration methods? 4 、 what factors are the main factors affecting the determination of crude fiber? 5. In the determination of crude fiber, why must we strictly control the experimental conditions? 6. What is the result of this method called crude fiber? The second module: enzymology experiment technology 1. Why does the enzyme specificity test do the reagent test? What might be the result of omitting this step? 2, what are the specificity of enzyme action? 3. Explain the function of reversible inhibitors and irreversible inhibitors What is the relationship between the inhibition degree of 4 and three reversible inhibitors and substrate concentration? 5. What are the molecular mechanisms leading to the loss of enzyme activity at high temperatures? How can we overcome this effect in the separation and purification of enzymes? 6, what other factors contribute to the decrease in enzyme activity? What is the molecular mechanism of the loss of enzyme activity resulting from 7 and pH values? 8, pH change caused by the loss of activity, pH recovery after the activity can also restore it? 9. Try to explain the physical meaning and biological significance of the Michaelis Menten constant Km 10. Why does the Michaelis constant Km be a characteristic constant of the