Guidelines for Blood Collection University of （大学采血指南）.pdfVIP

Department of Laboratory Animal Resources Guidelines for Blood Collection: Rodents and Rabbits CONTENTS Overview on Blood Sampling Collection Volumes Terminal Collections Anesthesia Methods Training Available Blood Collection in Mice Blood Collection in Rats Blood Collection in Rabbits Retro-Orbital Blood Collection Too much blood collected at any one time may cause hypovolemic shock, physiological stress and even death. If smaller volumes are collected too frequently, anemia may result. Collection Volumes The total blood volume can be calculated as approximately 6% of normal body weight. The estimated volume at exsanguination is approximately half of the total blood volume. For multiple blood draws separated by a period of weeks, a maximum of 1% of the animals body weight can be removed, i.e., 0.2 ml from a 20 gram mouse; 4 ml from a 400 gram rat, 40 ml from a 4 kg rabbit. A 14 day recovery period is needed for the average healthy adult animal to recover from this blood loss. Although the blood volume is restored within 24 hours after blood withdrawal, two weeks are needed for all constituents of the blood to return to normal. If less than the maximum amount of blood is withdrawn the animal will replace blood constituents at the rate of 1 ml/kg/day. By monitoring the hematocrit (packed cell volume=PCV) and/or hemoglobin of the animal, it is possible to evaluate whether the patient has sufficiently recovered from a single blood draw or multiple blood draws. After a sudden or acute blood loss, it takes up to 24 hours for the hematocrit and hemoglobin to reflect this loss. This means that after a 1% of body weight blood loss without fluid replacement, an animals hematocrit will not show a measurable drop for several hours, and will not stabilize for 24 hours. After 24