小麦TaYAB2基因的过量表达造成转基因拟南芥叶片近轴面-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2012, 38(11): 2042−2051 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.02042 小麦TaYAB2 基因的过量表达造成转基因拟南芥叶片近轴面特征趋向 远轴面 赵翔宇** 谢洪涛** 陈祥彬 王帅帅 张宪省* / / , 271018 : , YABBY TaYAB2, N C2C2 , C YABBY , AtYAB2 OsYAB2 RT-PCR , TaYAB2 , FIL/ YAB1YAB3KAN1 , TaYAB2 FIL/ YAB1YAB3KAN1 - YABBY - : -; ; TaYAB2; ; Ectopic Expression of TaYAB2, a Member of YABBY Gene Family in Wheat, Causes Partial Abaxialization of Adaxial Epidermises of Leaves in Arabidopsis ** ** * ZHAO Xiang-Yu , XIE Hong-Tao , CHEN Xiang-Bin, WANG Shuai-Shuai, and ZHANG Xian-Sheng State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China Abstract: Adaxial-abaxial polarity is an important feature of lateral organs. To study the molecular mechanism of polarity estab- lishment, we isolated a YABBY gene, designated TaYAB2, from the young leaves of wheat. TaYAB2 has a zinc finger-like domain in the N terminus and a YABBY domain in the C terminus. Sequence comparison showed that TaYAB2 is a putative member of the YABBY gene family in wheat. Further expression analysis indicated that TaYAB2 was widely expressed in the lateral organs of wheat. Ectopic expression of TaYAB2 in Arabidopsis caused the partial abaxialization of the adaxial epidermises of leaves with the promotion of the transcript leve