naturally occurring culturable aerobic gut flora of adult phlebotomus papatasi, vector of leishmania major in the old world天然可耕种的有氧肠道菌群的成年phlebotomus papatasi,向量利什曼虫主要的旧世界.pdfVIP

Naturally Occurring Culturable Aerobic Gut Flora of Adult Phlebotomus papatasi, Vector of Leishmania major in the Old World 1 3 1 1,2 Jaba Mukhopadhyay , Henk R. Braig , Edgar D. Rowton , Kashinath Ghosh * 1 Division of Entomology, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, 2 Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, 3 School of Biological Sciences, Bangor University, Bangor, Wales, United Kingdom Abstract Background: Cutaneous leishmaniasis is a neglected, vector-borne parasitic disease and is responsible for persistent, often disfiguring lesions and other associated complications. Leishmania, causing zoonotic cutaneous leishmaniasis (ZCL) in the Old World are mainly transmitted by the predominant sand fly vector, Phlebotomus papatasi. To date, there is no efficient control measure or vaccine available for this widespread insect-borne infectious disease. Methodology/Principal Findings: A survey was carried out to study the abundance of different natural gut flora in P. papatasi, with the long-term goal of generating a paratransgenic sand fly that can potentially block the development of Leishmania in the sand fly gut, thereby preventing transmission of leishmania in endemic disease foci. Sand flies, in particular, P. papatasi were captured from different habitats of various parts of the world. Gut microbes were cultured and identified using 16S ribosomal DNA analysis and a phylogenetic tree was constructed. We found variation in the species and abundance of gut flora in flies collected from different habitats. However, a few Gram-positive, nonpa