natural killer cells in obesity impaired function and increased susceptibility to the effects of cigarette smoke肥胖自然杀伤细胞功能受损,增加了对香烟烟雾的影响.pdfVIP

Natural Killer Cells in Obesity: Impaired Function and Increased Susceptibility to the Effects of Cigarette Smoke 1 2 3 4,5 Donal O’Shea , Tom J. Cawood , Cliona O’Farrelly , Lydia Lynch * 1 Department of Endocrinology, St. Vincent’s University Hospital, Dublin, Ireland, 2 Department of Endocrinology, Christchurch Hospital, Christchurch, New Zealand, 3 School of Biochemistry and Immunology and School of Medicine, Trinity College Dublin, Dublin, Ireland, 4 Oncology and Hematology, Beth Israel Deaconess Medical Centre, Harvard Medical School, Boston, Massachusetts, United States of America, 5 Obesity and Immunology, Education and Research Centre, St. Vincent’s University Hospital, Dublin, Ireland Abstract Background: Obese individuals who smoke have a 14 year reduction in life expectancy. Both obesity and smoking are independantly associated with increased risk of malignancy. Natural killer cells (NK) are critical mediators of anti-tumour immunity and are compromised in obese patients and smokers. We examined whether NK cell function was differentially affected by cigarette smoke in obese and lean subjects. 2 Methodology and Principal Findings: Clinical data and blood were collected from 40 severely obese subjects (BMI.40 kg/m ) and 20 lean healthy subjects. NK cell levels and function were assessed using flow cytometry and cytotoxicity assays. The effect of cigarette smoke on NK cell ability to kill K562 tumour cells was assessed in the presence or absence of the adipokines leptin and adiponectin. NK cell levels were significantly decreased in obese subjects