n-acetylcysteine increases the frequency of bone marrow pro-bpre-b cells, but does not reverse cigarette smoking-induced loss of this subset防治骨髓pro-bpre-b细胞的频率增加,但不反烟吸烟引起的损失的子集.pdfVIP

N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset 1 1 2 2,3 Victoria L. Palmer , Michele D. Kassmeier , James Willcockson , Mohammed P. Akhter , Diane M. 2 1 Cullen , Patrick C. Swanson * 1 Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, United States of America, 2 Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska, United States of America, 3 Osteoporosis Research Center, Creighton University School of Medicine, Omaha, Nebraska, United States of America Abstract Background: We previously showed that mice exposed to cigarette smoke for three weeks exhibit loss of bone marrow B cells at the Pro-B-to-pre-B cell transition, but the reason for this is unclear. The antioxidant N-acetylcysteine (NAC), a glutathione precursor, has been used as a chemopreventive agent to reduce adverse effects of cigarette smoke exposure on lung function. Here we determined whether smoke exposure impairs B cell development by inducing cell cycle arrest or apoptosis, and whether NAC treatment prevents smoking-induced loss of developing B cells. Methodology/Principal Findings: Groups of normal mice were either exposed to filtered room air or cigarette smoke with or without concomitant NAC treatment for 5 days/week for three weeks. Bone marrow B cell developmental subsets were enumerated, and sorted pro-B (B220+ + + 2 CD43 ) and pre-B (B220 CD43 ) cell fractions were analyze